Gao Jingru, Liu Qiang, Liu Wei, Jin Yan, Li Baoxin
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Analyst. 2021 Apr 26;146(8):2567-2573. doi: 10.1039/d1an00252j.
Both G-quadruplex (G4) and G-triplex (G3) can bind thioflavin T (ThT) to light up the fluorescence of ThT. G4/ThT and G3/ThT can be used as fluorescent indicators to construct a label-free molecular beacon (MB). In this work, we present a comparative perspective of G3/ThT-based MB and G4/ThT-based MB. The results showed that the G3/ThT-based MB had higher sensitivity and faster response speed than the G4/ThT-based MB. Furthermore, we systematically studied the effect of stem length and varying pairs on the response of the G3/ThT-based MB, and then proposed one rational design of the G3/ThT-based MB. This work demonstrates that the shorter G3 is more suitable for constructing the MB stem. This present work opens a promising way to develop a sensitive, simple and homogeneous biosensing method.
G-四链体（G4）和G-三链体（G3）均可与硫黄素T（ThT）结合，使ThT发出荧光。G4/ThT和G3/ThT可用作荧光指示剂来构建无标记分子信标（MB）。在这项工作中，我们对基于G3/ThT的分子信标和基于G4/ThT的分子信标进行了比较。结果表明，基于G3/ThT的分子信标比基于G4/ThT的分子信标具有更高的灵敏度和更快的响应速度。此外，我们系统地研究了茎长度和不同碱基对组合对基于G3/ThT的分子信标的响应的影响，然后提出了一种基于G3/ThT的分子信标的合理设计方案。这项工作表明，较短的G3更适合构建分子信标的茎。目前的这项工作为开发一种灵敏、简单且均相的生物传感方法开辟了一条有前景的道路。
