State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology - Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.
University of Chinese Academy of Sciences, Beijing, 10049, China.
Anal Bioanal Chem. 2022 Aug;414(20):6149-6156. doi: 10.1007/s00216-022-04180-7. Epub 2022 Jun 21.
Few reports are found working on the features and functions of the human telomere G-triplex (ht-G3) though the telomere G-quadruplex has been intensely studied and widely implemented to develop various biosensors. We herein report that ht-G3 lights up Thioflavin T (ThT) and establish a sensitive biosensing platform for RNA detection by introducing a target recycling strategy. An optimal condition was selected out for ht-G3 to promote ThT to generate a strong fluorescence. Accordingly, an ht-G3-based molecular beacon was successfully designed against the corresponding RNA sequence of the SARS-CoV-2 N-gene. The sensitivity for the non-amplified RNA target achieves 0.01 nM, improved 100 times over the conventional ThT-based method. We believe this ht-G3/ThT-based label-free strategy could be widely applied for RNA detection.
虽然端粒 G-四链体已被深入研究并广泛应用于开发各种生物传感器，但很少有报道涉及人类端粒 G-三链体（ht-G3）的特征和功能。本文报道了 ht-G3 可使噻唑蓝（ThT）发荧光，并通过引入靶标循环策略，建立了一种用于 RNA 检测的灵敏生物传感平台。选择了最佳条件以促进 ht-G3 使 ThT 产生强荧光。因此，成功设计了针对 SARS-CoV-2 N 基因相应 RNA 序列的基于 ht-G3 的分子信标。与传统基于 ThT 的方法相比，对未扩增的 RNA 靶标的灵敏度提高了 100 倍，达到 0.01 nM。我们相信，这种基于 ht-G3/ThT 的无标记策略可广泛应用于 RNA 检测。
