Wang Ke, Qin Zhen, Wu Shiyuan, Zhao Pengyu, Zhen Chaoying, Gao Haiyan
School of Life Sciences, Shanghai University, Shanghai 200444, China.
J Agric Food Chem. 2021 May 5;69(17):5267-5278. doi: 10.1021/acs.jafc.1c00640. Epub 2021 Apr 26.
is commonly used as a biocontrol bacterium owing to its strong antifungal activity, broad-spectrum inhibition, and general safety. In this study, the inhibitory effects of volatile organic compounds (VOCs) produced by CF-3 on , a major destructive phytopathogen of litchi anthracnose, were analyzed using proteomics and transcriptomics. Differentially expressed genes (DEGs) and proteins (DEPs) indicated that the inhibition of by CF-3 VOCs downregulated the expression of genes related to cell membrane fluidity, cell wall integrity, energy metabolism, and production of cell wall-degrading enzymes. Particularly, those important DEGs and DEPs related to the ergosterol biosynthetic and biosynthesis of unsaturated fatty acids are most significantly influenced. 2,4-di--butylphenol, a characteristic component of CF-3 VOCs, also showed a similar effect on . Our results provide a theoretical basis for the potential application of CF-3 in the postharvest protection of fruits and vegetables.
由于其强大的抗真菌活性、广谱抑制作用和普遍安全性，通常被用作生防细菌。在本研究中，使用蛋白质组学和转录组学分析了CF-3产生的挥发性有机化合物（VOCs）对荔枝炭疽病的主要破坏性植物病原体的抑制作用。差异表达基因（DEGs）和蛋白质（DEPs）表明，CF-3 VOCs对其的抑制作用下调了与细胞膜流动性、细胞壁完整性、能量代谢和细胞壁降解酶产生相关的基因表达。特别是，那些与麦角固醇生物合成和不饱和脂肪酸生物合成相关的重要DEGs和DEPs受到的影响最为显著。CF-3 VOCs的特征成分2,4-二叔丁基苯酚对其也表现出类似的作用。我们的结果为CF-3在果蔬采后保鲜中的潜在应用提供了理论依据。
