Fan Yongchao, Lv Mengmeng, Xue Yuan, Li Jing, Wang Erkang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.
University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.
Anal Chem. 2021 May 4;93(17):6873-6880. doi: 10.1021/acs.analchem.1c00967. Epub 2021 Apr 25.
A highly fluorescent emission reaction between terephthalic acid (PTA) and ascorbic acid (AA) via simple control of the reaction temperature was first revealed with the detailed formation mechanism and various characterizations including electron paramagnetic resonance and mass spectrometry. Based on the AA-responsive emission, the alkaline phosphatase (ALP) triggered the transformation of l-ascorbic acid 2-phosphate trisodium salt to AA was integrated with the present system for developing a sensitive, selective, and universal platform. The monitoring of the activity of ALP and the fabrication of ALP-based enzyme-linked immunoassay (ELISA) with carcinoembryonic antigen (CEA) as the model target was performed. The fluorescence intensity correlated well to the CEA concentration in the ranges of 0.25-30 ng/mL, with a detection limit of 0.08 ng/mL. Such a facile protocol based on the fluorescent reaction between PTA and AA without the assistance of catalysis of nanomaterials avoided the laborious synthesis procedure and provided a direct strategy for the early clinical diagnosis coupled with ALP-related catalysis.
首次通过简单控制反应温度揭示了对苯二甲酸（PTA）与抗坏血酸（AA）之间高度荧光发射反应，并详细阐述了其形成机制，还进行了包括电子顺磁共振和质谱在内的各种表征。基于对AA响应的发射，将碱性磷酸酶（ALP）触发的L-抗坏血酸2-磷酸三钠盐向AA的转化与本系统相结合，以开发一个灵敏、选择性好且通用的平台。进行了碱性磷酸酶活性监测以及以癌胚抗原（CEA）为模型靶点的基于碱性磷酸酶的酶联免疫吸附测定（ELISA）的构建。在0.25 - 30 ng/mL范围内，荧光强度与CEA浓度具有良好的相关性，检测限为0.08 ng/mL。这种基于PTA与AA之间荧光反应且无需纳米材料催化辅助的简便方案，避免了繁琐的合成过程，并为结合ALP相关催化的早期临床诊断提供了直接策略。
