School of Chemistry and Pharmaceutical Sciences, State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China.
Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, China.
Biosensors (Basel). 2022 Oct 6;12(10):830. doi: 10.3390/bios12100830.
Copper peroxide/zeolitic imidazolate framework/polydopamine nanoparticles (CP/ZIF-8/PDA)-based fluorescence-linked immunosorbent assay (FLISA) was designed for the sensitive and high-throughput determination of carcinoembryonic antigen (CEA) by self-supplied HO generation. Specifically, the CEA aptamer was modified on the surface of CP/ZIF-8/PDA to form an immunoprobe. The structures of CP and ZIF-8 could be broken under acidic conditions, and produced the Cu and HO due to the dissociation the CP. A subsequent Fenton-type reaction of Cu and HO generated hydroxyl radical (·OH). o-phenylenediamine (OPD) was oxidized by the ·OH to form 2, 3-diaminophenazine (DPA) with a significant fluorescence signal. CP/ZIF-8/PDA could be used as an efficient Fenton-type reactant to generate a large amount of ·OH to promote OPD oxidation. The sensitive detection of CEA could be realized. Under optimal conditions, the FLISA platform displayed a linear detection range from 0.01 to 20 ng mL with a detection limit of 7.6 pg mL for CEA. This strategy has great application potential for sensitive and high-throughput determination for other biomarkers in the field of biomedicine.
基于过氧氧化铜/沸石咪唑酯骨架/聚多巴胺纳米粒子（CP/ZIF-8/PDA）的荧光联免疫吸附测定法（FLISA）通过自供应 HO 生成设计用于灵敏和高通量测定癌胚抗原（CEA）。具体而言，CEA 适体被修饰在 CP/ZIF-8/PDA 的表面上以形成免疫探针。在酸性条件下，CP 和 ZIF-8 的结构会被破坏，并由于 CP 的解离而产生 Cu 和 HO。随后，Cu 和 HO 发生芬顿型反应生成羟基自由基（·OH）。邻苯二胺（OPD）被·OH 氧化生成具有显著荧光信号的 2,3-二氨基吩嗪（DPA）。CP/ZIF-8/PDA 可用作有效的芬顿型反应物以生成大量的·OH 以促进 OPD 氧化。可以实现对 CEA 的灵敏检测。在最佳条件下，FLISA 平台显示出从 0.01 到 20ng/mL 的线性检测范围，CEA 的检测限为 7.6pg/mL。该策略在生物医学领域中对其他生物标志物进行灵敏和高通量测定具有巨大的应用潜力。
