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潜在食物垃圾降解菌在恶劣条件下的效率。

The efficiency of potential food waste-degrading bacteria under harsh conditions.

机构信息

Department of Environmental Energy Engineering, Graduate School, Kyonggi University, Suwon, South Korea.

Department of Environmental Energy Engineering, College of Creative Engineering, Kyonggi University, Suwon, South Korea.

出版信息

J Appl Microbiol. 2022 Jan;132(1):340-350. doi: 10.1111/jam.15119. Epub 2021 Sep 18.

Abstract

AIMS

Investigate the impact of highly adapted bacterial strains and their ability in waste degradation under a wide range of temperatures.

METHODS AND RESULTS

Bacteria isolated from soil and food waste were grown in various media under fluctuated temperatures. After screening for organic compound degradation, the seven strongest bacterial strains have been selected for further experiments. Their enzyme activities were expressed in terms of the size of the hydrolysis zone in a wide temperature range of 2·5-70°C. The enzyme production assay was carried out for each protease, cellulase and amylase. The waste degradation was determined with a maximum 80% decrease in the volume of food waste in 21 days compared to the control in lab scale with enriched bacterial cultures and soil bacteria as additives at room temperature around 18-20°C.

CONCLUSION

These seven bacteria are promising candidates for food waste biodegradation in composting especially in the winter without heating expense for maintaining ambient temperature.

SIGNIFICANCE AND IMPACT OF THE STUDY

It is necessary to coax the uncultured bacteria from the various environments into the laboratory for investigating their valuable functions. Herein, using enrichment culture of consortium and additive of soil has illustrated the significant mean in food waste degradation.

摘要

目的

研究高度适应的细菌菌株及其在广泛温度范围内进行废物降解的能力的影响。

方法和结果

从土壤和食物废物中分离出的细菌在不同的培养基中在波动的温度下生长。经过对有机化合物降解的筛选,选择了 7 株最强的细菌菌株进行进一步的实验。它们的酶活性在 2.5-70°C 的宽温度范围内用水解带的大小表示。对每个蛋白酶、纤维素酶和淀粉酶进行了酶生产测定。通过在实验室规模的富集细菌培养物和土壤细菌作为添加剂,在室温约 18-20°C 下,与对照相比,在 21 天内使食物废物的体积减少了 80%,从而确定了废物的降解。

结论

这些 7 种细菌是堆肥中进行食物废物生物降解的有前途的候选者,特别是在冬季无需加热费用来维持环境温度。

研究的意义和影响

有必要将各种环境中的未培养细菌诱导入实验室,以研究它们的有价值的功能。在这里,使用共培养物的富集培养和土壤添加剂说明了在食物废物降解方面的重要意义。

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