Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Bioresour Technol. 2021 Nov;340:125635. doi: 10.1016/j.biortech.2021.125635. Epub 2021 Jul 22.
This study aims to construct a high-temperature-resistant microbial consortium to effectively degrade oily food waste by Fed-in-situ biological reduction treatment (FBRT). Oil degrading bacteria were screened under thermophilic conditions of mineral salt medium with increased oil content. The oil degradation and emulsification ability of each stain was evaluated and their synergetic improvement was further confirmed. Consortium of Bacillus tequilensis, Bacillus licheniformis, Bacillus sonorensis and Ureibacillus thermosphaericus was selected and applicated as bacterial agents in FBRT under 55 °C. Changes in pH, moisture, bacterial community and key components of food waste were monitored for 5 days during processing. Facilitated by the bacterial consortium, FBRT gave superior total mass reduction (86.61 ± 0.58% vs. 67.25 ± 1.63%) and non-volatile solids reduction (65.91 ± 1.53% vs. 28.53 ± 2.29%) compared with negative control, the feasibility and efficiency of present FBRT providing a promising in-situ disposal strategy for rapid reduction of oily food waste.
本研究旨在构建一种耐高温微生物联合体,通过 Fed-in-situ 生物还原处理(FBRT)有效降解含油食物废物。在矿物盐培养基中增加油含量的高温条件下筛选出了石油降解细菌。评估了每种菌株的油降解和乳化能力,并进一步证实了它们的协同提高。选择了巴氏芽孢杆菌、地衣芽孢杆菌、苏云金芽孢杆菌和嗜热解淀粉芽孢杆菌的联合体,并在 55°C 下作为细菌剂应用于 FBRT。在处理过程中,监测了 5 天内 pH 值、水分、细菌群落和食物废物关键成分的变化。在细菌联合体的促进下,FBRT 的总质量减少率(86.61±0.58%比67.25±1.63%)和非挥发性固体减少率(65.91±1.53%比28.53±2.29%)均优于阴性对照,表明目前的 FBRT 具有可行性和效率,为快速减少含油食物废物提供了一种有前景的原位处理策略。