Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, New Jersey 08901, USA.
Department of Food Science and Engineering, Ningbo University, Ningbo 315211, Zhejiang, China.
Food Funct. 2021 Apr 26;12(8):3516-3526. doi: 10.1039/d1fo00126d.
Nobiletin is abundant in citrus peels and demonstrates good anti-obesity bioactivity. However, its anti-obesity mechanisms still remain unclear. This study aims to explore the bidirectional interaction between nobiletin and gut microbiota in mice fed with a high-fat diet. For the colonic bioconversion, more demethylated metabolites with higher biological activity were found in feces than nobiletin in the 48 h excretion study and 8 week consecutive dosing study. Moreover, long-term oral intake of nobiletin would modify the gut microbiota with improved demethylation ability and enhanced production of short chain fatty acids. The comparison of metabolite profiles in mouse liver and feces indicated that gut microbiota might have a higher biotransformation activity on nobiletin than the host. Two bacteria at the genus level, Allobaculum and Roseburia, remained enriched by nobiletin after the 4- and 8-week feedings. They might correlate with the enhanced nobiletin biotransformation and actively contribute to the health benefits of nobiletin in vivo. These results suggested that the bidirectional interaction of nobiletin and gut microbiota played an important role on the anti-obesity effect of nobiletin.
川陈皮素在柑橘皮中含量丰富，具有良好的抗肥胖生物活性。然而，其抗肥胖机制仍不清楚。本研究旨在探索高脂肪饮食喂养小鼠中川陈皮素与肠道微生物群的双向相互作用。在结肠生物转化方面，与川陈皮素相比，在 48 小时排泄研究和 8 周连续给药研究中，粪便中发现了更多具有更高生物活性的去甲基化代谢物。此外，长期口服川陈皮素会修饰肠道微生物群，提高去甲基化能力，增强短链脂肪酸的产生。对小鼠肝和粪便中代谢物谱的比较表明，肠道微生物群对川陈皮素的生物转化活性可能高于宿主。两种细菌在属水平上，即 Allobaculum 和 Roseburia，在 4 周和 8 周的喂养后仍被川陈皮素富集。它们可能与增强的川陈皮素生物转化有关，并积极促进川陈皮素在体内的健康益处。这些结果表明，川陈皮素与肠道微生物群的双向相互作用对川陈皮素的抗肥胖作用起着重要作用。
