Dep. of Plant Pathology & Microbiology, Texas A&M Univ., College Station, TX, 77843, USA.
USDA-ARS Southern Plains Agricultural Research Center, College Station, TX, 77845, USA.
Plant Genome. 2021 Jul;14(2):e20097. doi: 10.1002/tpg2.20097. Epub 2021 Apr 26.
Anthracnose disease of sorghum is caused by Colletotrichum sublineola, a filamentous fungus. The genetic basis of resistance to anthracnose in sorghum is largely unclear, especially in Senegalese sorghum germplasm. In this study, 163 Senegalese sorghum accessions were evaluated for response to C. sublineola, and a genome-wide association study (GWAS) was performed to identify genetic variation associated with response to C. sublineola using 193,727 single nucleotide polymorphisms (SNPs) throughout the genome. Germplasm diversity analysis showed low genetic diversity and slow linkage disequilibrium (LD) decay among the Senegalese accessions. Phenotypic analysis resulted in relatively low differences to C. sublineola among the tested population. Genome-wide association study did not identify any significant association based on a strict threshold for the number of SNPs available. However, individual analysis of the top eight SNPs associated with relative susceptibility and resistance identified candidate genes that have been shown to play important roles in plant stress tolerance in previous studies. This study identifies sorghum genes whose annotated properties have known roles in host defense and thus identify them as candidates for use in breeding for resistance to anthracnose.
高粱炭疽病由胶孢炭疽菌引起，胶孢炭疽菌是一种丝状真菌。高粱对炭疽病的抗性遗传基础在很大程度上尚不清楚，特别是在塞内加尔高粱种质资源中。在这项研究中，对 163 份塞内加尔高粱品系进行了炭疽病的反应评估，并进行了全基因组关联研究 (GWAS)，利用全基因组的 193727 个单核苷酸多态性 (SNP) 鉴定与炭疽病反应相关的遗传变异。种质资源多样性分析表明，塞内加尔品系之间遗传多样性低，连锁不平衡 (LD) 衰减缓慢。表型分析表明，在测试的种群中，炭疽病的差异相对较低。全基因组关联研究没有发现任何基于可用 SNP 数量的严格阈值的显著关联。然而，对与相对易感性和抗性相关的前 8 个 SNP 进行的单独分析，确定了候选基因，这些基因在以前的研究中被证明在植物胁迫耐受中发挥着重要作用。这项研究确定了高粱基因，其注释特性已知在宿主防御中具有重要作用，因此将其鉴定为用于炭疽病抗性育种的候选基因。
