Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843, USA.
USDA-ARS Plant Science Research Unit, St. Paul, MN, 55108, USA.
Sci Rep. 2022 Jul 29;12(1):13025. doi: 10.1038/s41598-022-16844-6.
Colletotrichum sublineola is a destructive fungal pathogen that causes anthracnose in sorghum. Senegalese sorghum germplasm is currently being considered as an option of sources for genetic resistance. In a recent study, Senegalese sorghum accessions were evaluated for response to a mixture of Texas isolates of C. sublineola at the 8-leaf stage in the greenhouse. As a comparison, 159 Senegalese sorghum accessions at the 1-leaf developmental stage were evaluated against a single Texas isolate of C. sublineola (FSP53) using an excised-leaf assay. A genome-wide association study (GWAS) was conducted based on the phenotypic data acquired to discover genetic variation associated with response to C. sublineola using 193,727 single nucleotide polymorphisms (SNPs) throughout the genome. Sorghum seedlings tended to be more resistant when compared with sorghum plants inoculated at the 8-leaf stage in the greenhouse in previous experiments. Based on the highest score evaluated in the 1-leaf developmental stage excised leaf assay for each accession, 16 accessions were labeled as susceptible. GWAS identified the SNP locus S01_72868925 that is associated with protein kinase domain // Leucine rich repeat N-terminal domain at a level of confidence that surpassed Bonferroni correction. Along with the SNP locus S01_72868925, other top SNP loci were also associated with genes that are known to play critical roles in plant defense or plant stress responses.
黑线炭疽菌是一种具有破坏性的真菌病原体，可导致高粱炭疽病。塞内加尔高粱种质资源目前被认为是遗传抗性的来源之一。在最近的一项研究中，温室 8 叶期评估了塞内加尔高粱品系对德克萨斯州混合分离株 C. sublineola 的反应。作为比较，在 1 叶期发育阶段，使用离体叶片测定法，用德克萨斯州的单一 C. sublineola 分离株（FSP53）评估了 159 份塞内加尔高粱品系。基于获得的表型数据进行了全基因组关联研究（GWAS），以发现与对 C. sublineola 反应相关的遗传变异，使用整个基因组中的 193,727 个单核苷酸多态性（SNP）。与以前在温室中 8 叶期接种的高粱植物相比，高粱幼苗的抗性更强。基于每个品系在 1 叶期发育阶段离体叶片测定中评估的最高得分，有 16 个品系被标记为易感。GWAS 鉴定出与蛋白激酶结构域//富含亮氨酸重复 N 端结构域相关的 SNP 位点 S01_72868925，置信水平超过了 Bonferroni 校正。除了 SNP 位点 S01_72868925 外，其他顶级 SNP 位点还与已知在植物防御或植物应激反应中发挥关键作用的基因相关。
