Oncology Disease Research Unit, Children's Hospital of Mexico, Federico Gomez, Mexico City, Mexico.
Hemato-Oncology Service, Moctezuma Children's Hospital, Mexico City, Mexico.
Pediatr Hematol Oncol. 2021 Aug;38(5):456-470. doi: 10.1080/08880018.2020.1871139. Epub 2021 Apr 26.
Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer, constituting 80% of all acute leukemias in minors. Despite the increase in the success of therapies, disease-free survival is over 80% in most cases. For the remaining 20% of patients, new strategies are needed to allow us to know and select those at greatest risk of relapse. We evaluated by immunohistochemistry the expression of the transcription factor YY1 and found that it is overexpressed in peripheral blood leukemia cells of pediatric patients with ALL with Pro-B and T phenotype compared to control samples. Over expression of YY1 was associated with a significantly lower chance of survival. We also evaluated by RT-PCR in bone marrow samples from ALL pediatric patients the association of YY1 expression with the percentage of blasts. High levels of YY1 were present in samples with higher percent of blasts in these patients. In addition, ALL pediatric patients with a poor response to therapy had higher levels at the nuclear level of YY1 than those who responded well to chemotherapy. In conclusion, our data suggest that YY1 could serve in pediatric ALL as markers of evolution and response for this disease, mainly in patients with pro-B and T immunophenotype. It is also suggested that YY1 is implicated in the expanse of blast in these patients.
急性淋巴细胞白血病（ALL）是最常见的儿科癌症，占未成年人所有急性白血病的 80%。尽管治疗成功率有所提高，但大多数情况下无病生存率超过 80%。对于剩下的 20%的患者，需要新的策略来让我们了解并选择那些复发风险最大的患者。我们通过免疫组织化学评估了转录因子 YY1 的表达，发现与对照样本相比，具有 Pro-B 和 T 表型的儿科 ALL 患者外周血白血病细胞中 YY1 表达过度。YY1 的过度表达与存活率显著降低相关。我们还通过 RT-PCR 评估了来自 ALL 儿科患者的骨髓样本中 YY1 表达与原始细胞百分比的关联。在这些患者中，原始细胞百分比较高的样本中存在高水平的 YY1。此外，对治疗反应不佳的 ALL 儿科患者的 YY1 核水平高于对化疗反应良好的患者。总之，我们的数据表明，YY1 可作为儿科 ALL 疾病演变和反应的标志物，主要针对具有 Pro-B 和 T 免疫表型的患者。还表明 YY1 参与了这些患者原始细胞的扩增。
