Eamens G J, Kirkland P D, Turner M J, Gardner I A, White M P, Hornitzky C L
Department of Agriculture, Central Veterinary Laboratory, Glenfield, New South Wales.
Aust Vet J. 1988 Apr;65(4):120-3. doi: 10.1111/j.1751-0813.1988.tb14430.x.
Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.
通过使用牛鼻甲细胞或牛肺细胞的琼脂糖覆盖法，多杀性巴氏杆菌的产毒株很容易与非产毒株区分开来。年轻且密集汇合的细胞最适合此检测。区分产毒株所需的潜伏期取决于单层细胞的汇合度，而汇合度受接种率、细胞传代水平和覆盖前的生长时间影响。琼脂糖覆盖法正确鉴定了11株多杀性巴氏杆菌参考菌株中的11株，48至65小时后单层细胞出现明显的细胞毒性变化。新南威尔士州2个养猪场的地方性萎缩性鼻炎疫情与多杀性巴氏杆菌产毒D型菌株的分离有关。
