Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, China.
Research Center of Carcinogenesis and Targeted Therapy, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
Aging (Albany NY). 2021 Apr 26;13(9):12607-12630. doi: 10.18632/aging.202937.
Novel biomarkers are needed to accelerate the diagnosis and treatment of endometriosis. We performed RNA sequencing to explore the expression profiles of exosomal circular RNAs (circRNAs), microRNAs (miRNAs) and mRNAs in patients with ovarian endometriomas, eutopic endometria and normal endometria. Differentially expressed genes between the different pairs of groups were analyzed and functionally annotated. Then, miRNA-target RNA pairs were identified, competing endogenous RNA (ceRNA) scores were calculated, gene expression characteristics were determined, and these parameters were used to construct an exosomal ceRNA network. We identified 36 candidate hub genes with high degrees of gene connectivity. We also topologically analyzed the ceRNA network to obtain a hub ceRNA network of circRNAs with the highest closeness and ceRNA efficiency. Twelve genes overlapped between the 36 candidate hub genes and the genes in the hub ceRNA network. These 12 genes were considered to be exosomal RNA-based biomarkers, and circ_0026129/miRNA-15a-5p/ATPase H+ transporting V1 subunit A () were at the center of the ceRNA network. By determining the exosomal RNA expression profiles of endometriosis patients and constructing a circRNA-associated ceRNA network, these findings provide insight into the molecular pathways of endometriosis and new resources for its diagnosis and treatment.
需要新的生物标志物来加速内异症的诊断和治疗。我们进行了 RNA 测序，以探索卵巢子宫内膜异位症、在位子宫内膜和正常子宫内膜患者中细胞外囊泡环状 RNA（circRNA）、微小 RNA（miRNA）和信使 RNA（mRNA）的表达谱。分析了不同组对之间差异表达的基因，并进行了功能注释。然后，鉴定 miRNA 靶 RNA 对，计算竞争内源性 RNA（ceRNA）评分，确定基因表达特征，并使用这些参数构建外泌体 ceRNA 网络。我们鉴定出 36 个候选枢纽基因，这些基因的基因连接度很高。我们还对 ceRNA 网络进行拓扑分析，获得具有最高紧密性和 ceRNA 效率的环状 RNA 枢纽 ceRNA 网络。在 36 个候选枢纽基因和枢纽 ceRNA 网络中的基因之间有 12 个重叠基因。这些 12 个基因被认为是基于外泌体 RNA 的生物标志物，circ_0026129/miRNA-15a-5p/ATPase H+ transporting V1 subunit A () 位于 ceRNA 网络的中心。通过确定内异症患者的外泌体 RNA 表达谱并构建环状 RNA 相关的 ceRNA 网络，这些发现为内异症的分子途径提供了深入了解，并为其诊断和治疗提供了新的资源。
