College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.
Laboratory of Enzyme Preparation, Hebei Research Institute of Microbiology, Baoding, China.
PLoS One. 2021 Apr 26;16(4):e0250675. doi: 10.1371/journal.pone.0250675. eCollection 2021.
Insect microbial symbioses play a critical role in insect lifecycle, and insect gut microbiome could be influenced by many factors. Studies have shown that host diet and taxonomy have a strong influence on insect gut microbial community. In this study, we performed sequencing of V3-V4 region of 16S rRNA gene to compare the composition and diversity of 12 Ensifera from 6 provinces of China. Moreover, the influences of feeding habits and taxonomic status of insects on their gut bacterial community were evaluated, which might provide reference for further application research. The results showed that Proteobacteria (45.66%), Firmicutes (34.25%) and Cyanobacteria (7.7%) were the predominant bacterial phyla in Ensifera. Moreover, the gut bacterial community composition of samples with different feeding habits was significantly different, which was irrespective of their taxa. The highest diversity of gut bacteria was found in the omnivorous Ensifera. Furthermore, common and unique bacteria with biomarkers were found based on the dietary characteristics of the samples. However, the bacterial community structure of the Ensifera samples was significantly different from that of Caelifera. Therefore, we concluded that feeding habits and taxonomic status jointly affect the gut bacterial community composition of the samples from Orthoptera. However, the influence of feeding habit dominates when taxonomy category below the suborder level. In addition, the dominant, common and unique bacterial community structure could be used to predict the contrastic feeding habits of insects belonging to Ensifera.
昆虫微生物共生在昆虫生命周期中起着关键作用，昆虫肠道微生物组可能受到许多因素的影响。研究表明，宿主饮食和分类对昆虫肠道微生物群落有很强的影响。在这项研究中，我们对来自中国 6 个省份的 12 种直翅目昆虫的 16S rRNA 基因 V3-V4 区进行了测序，以比较它们的组成和多样性。此外，还评估了昆虫的摄食习性和分类地位对其肠道细菌群落的影响，这可能为进一步的应用研究提供参考。结果表明，直翅目昆虫肠道细菌的主要菌群为变形菌门（45.66%）、厚壁菌门（34.25%）和蓝细菌门（7.7%）。此外，不同摄食习性的样本肠道细菌群落组成存在显著差异，而与分类无关。杂食性直翅目昆虫的肠道细菌多样性最高。此外，根据样本的饮食特征，发现了具有生物标志物的共有和特有细菌。然而，直翅目昆虫样本的细菌群落结构与长翅目昆虫样本的结构有显著差异。因此，我们得出结论，摄食习性和分类地位共同影响直翅目昆虫样本的肠道细菌群落组成。然而，当分类单元低于亚目水平时，摄食习性的影响占主导地位。此外，优势、共有和特有细菌群落结构可用于预测直翅目昆虫的摄食习性。
