乙醇对培养的交感神经细胞的影响。

Effect of ethyl alcohol on sympathetic nerve cells in cultures.

作者信息

Eränkö L, Eränkö O, Nikkinen A

出版信息

Med Biol. 1977 Oct;55(5):277-83.

Abstract

Sympathetic chain ganglia of newborn rats were cultured in Rose chambers and their structure was registered, examined and photographed with a phase contrast microscope. When 0.5% (v/v) (86 mM) ethanol was present in the culture medium from the start of culture, the nerve cell bodies appeared similar to those in control cultures for at least a week and there was a normal outgrowth of a nerve fibre network. Higher concentrations had a progessively deleterious effect, 1% (171 mM) ethanol causing the appearance of degenerating cells and 2% (343 mM) ethanol killing all the nerve cells in 4 days. Some nerve cells survived 4 days in the presence of 1.5% (275 mM) ethanol in the culture medium, although most cells died in 2 days. When ethanol was added to cultures grown for 7 days in a control medium, concentration of 0.5% ethanol in the medium had no apparent effect, while most nerve cells died within a week in 1% ethanol, and all cells were dead already after 24 hours in 2% ethanol. It is concluded that ethanol exhibits a remarkably low toxicity towards living nerve cells.

摘要

将新生大鼠的交感神经链神经节培养于罗斯小室中，并用相差显微镜对其结构进行记录、检查和拍照。从培养开始就在培养基中加入0.5%（体积/体积）（86 mM）乙醇时，神经细胞体至少一周内看起来与对照培养物中的相似，并且有正常的神经纤维网络长出。更高浓度则有逐渐有害的作用，1%（171 mM）乙醇导致出现退化细胞，2%（343 mM）乙醇在4天内杀死所有神经细胞。在培养基中有1.5%（275 mM）乙醇的情况下，一些神经细胞存活了4天，尽管大多数细胞在2天内死亡。当将乙醇添加到在对照培养基中培养7天的培养物中时，培养基中0.5%乙醇浓度没有明显影响，而在1%乙醇中大多数神经细胞在一周内死亡，在2%乙醇中所有细胞在24小时后就已死亡。得出的结论是，乙醇对活神经细胞表现出极低的毒性。