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[二、器官培养和移植中大鼠颈上神经节细胞的超微结构]

[II. Ultrastructure of rat superior cervical ganglion cells in organ culture and grafts].

作者信息

Coujard M F

出版信息

Z Mikrosk Anat Forsch. 1978;92(4):655-74.

PMID:749395
Abstract

Ultrastructural modifications of neurons in organ cultures were more and less obvious, while remaining always identical in cultures at the same age. Between 5 and 6 days after culture, neurons showed numerous dense bodies, nuclear pockets, sparse Nissl's bodies broken into fragments, bundles of microfilaments, elongated mitochondria and a slightly distended Golgi apparatus. Sometimes, the nucleus was indented and the nucleolus was shaded off. S.I.F. cells had indented nucleus, dense bodies and bundles of microfibrils. Neurons from direct grafts presented modifications similar to those observed in cultures at short time intervals. Then the breaking up of Nissl's bodies and mitochondria and the hyalin transformation of dense bodies took place. A restoration, at first involving the nucleus, could occur in relation with immunological conditions. The evolution of S.I.F. cells was quite comparable to that of neurons. In either case, features of cellular degeneracy were always little marked and fugitive.

摘要

器官培养中神经元的超微结构改变或多或少较为明显,而在相同年龄的培养物中始终保持一致。培养5至6天后,神经元呈现出大量致密小体、核袋、破碎成碎片的稀疏尼氏体、微丝束、细长的线粒体以及略微扩张的高尔基体。有时,细胞核有凹陷,核仁模糊不清。S.I.F.细胞有凹陷的细胞核、致密小体和微原纤维束。直接移植的神经元呈现出与短时间间隔培养中观察到的类似改变。然后尼氏体和线粒体破裂,致密小体发生透明变性。起初涉及细胞核的恢复可能与免疫状况有关。S.I.F.细胞的演变与神经元的演变相当。在任何一种情况下,细胞退化的特征总是不太明显且短暂的。

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