Souza Matheus Albino, Palhano Huriel Scartazzini, Macci Dierro, Broch Gabriélli Moraes, Tissiani Luanda, Rauber Bárbara Facco, Cecchin Doglas, Bervian Juliane, de Almeida Gomes Brenda Paula Figueiredo, Gabrielli Ezequiel Santin
School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil.
School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
Aust Endod J. 2021 Dec;47(3):531-537. doi: 10.1111/aej.12517. Epub 2021 Apr 26.
This study evaluated the influence of ultrasonic activation (US) on the antimicrobial activity of a new final irrigant containing glycolic acid (GA). Extracted teeth were used, being 70 to counting of colony-forming units (CFUs) and 35 to confocal laser scanning microscopy. Samples were inoculated with Enterococcus faecalis and divided into 7 groups: distilled water + US; 17% EDTA; Qmix; 17% GA; 17% EDTA + US; QMix + US; and 17% GA + US and kept in contact with test solution for 1 min in the groups with or no US. In the CFUs, the highest bacterial reduction was observed in QMix + US group, followed by QMix and GA + US. In the confocal evaluation, the lowest number of viable cells was observed in EDTA + US, with no statistical difference from QMix, QMix + US and GA + US (P > 0.05). The use of US improves the antimicrobial activity of EDTA and GA, being statistically different from the isolated use of these final irrigants in both evaluation tests.
本研究评估了超声激活（US）对含乙醇酸（GA）的新型终末冲洗剂抗菌活性的影响。使用拔除的牙齿，70颗用于菌落形成单位（CFU）计数，35颗用于共聚焦激光扫描显微镜检查。样本接种粪肠球菌后分为7组：蒸馏水+超声；17%乙二胺四乙酸（EDTA）；Qmix；17%GA；17%EDTA+超声；QMix+超声；17%GA+超声，在有或无超声的组中与测试溶液接触1分钟。在CFU计数中，QMix+超声组细菌减少最多，其次是QMix和GA+超声组。在共聚焦评估中，EDTA+超声组活细胞数量最少，与QMix、QMix+超声和GA+超声组无统计学差异（P>0.05）。超声的使用提高了EDTA和GA的抗菌活性，在两项评估测试中与单独使用这些终末冲洗剂相比有统计学差异。
