Souza Matheus A, Bischoff Karolina F, Rigo Bárbara D C, Piuco Larissa, Didoné Ana V L, Bertol Charise D, Rossato-Grando Luciana G, Bervian Juliane, Cecchin Doglas
School of Dentistry, University of Passo Fundo, Passo Fundo, Brazil.
Institute of Biological Sciences, University of Passo Fundo, Passo Fundo, Brazil.
Aust Endod J. 2021 Dec;47(3):423-428. doi: 10.1111/aej.12494. Epub 2021 Mar 8.
The aim of the study was to evaluate the cytotoxicity of different glycolic acid concentrations (GA) and its effects on dentinal microhardness. Cytotoxicity was evaluated after inoculation of test irrigants in the lymphocyte primary culture for 3 min. The tested substances were distilled water(DW); 17% EDTA; QMix; 10% GA; 17% GA; and 25% GA. Counting of total, live and dead cells was performed, obtaining the average percentage of dead cells of each group. For microhardness evaluation, 60 root dentin samples were divided into the same tested groups (n = 10) and immersed in test irrigants for 3 min. Dentin microhardness was evaluated by Vicker test. Specific statistical analysis was made in both tests. Results showed significant lower cytotoxicity for QMix and 10% GA (P < 0.05). Moreover, all test irrigants presented similar values of microhardness than the control group (P > 0.05). In conclusion, lower GA concentration can be an alternative for final irrigation on endodontics.
本研究的目的是评估不同浓度乙醇酸（GA）的细胞毒性及其对牙本质显微硬度的影响。在淋巴细胞原代培养物中接种测试冲洗剂3分钟后评估细胞毒性。测试物质为蒸馏水（DW）；17%乙二胺四乙酸（EDTA）；QMix；10%GA；17%GA；和25%GA。对总细胞、活细胞和死细胞进行计数，得出每组死细胞的平均百分比。为了评估显微硬度，将60个牙根牙本质样本分为相同的测试组（n = 10），并在测试冲洗剂中浸泡3分钟。通过维氏试验评估牙本质显微硬度。两项测试均进行了特定的统计分析。结果显示，QMix和10%GA的细胞毒性显著较低（P < 0.05）。此外，所有测试冲洗剂的显微硬度值与对照组相似（P > 0.05）。总之，较低浓度的GA可作为牙髓病学最终冲洗的替代方法。
