Maconi E, Griffini A, Cavazzoni V, Aragozzini F
Dipartmento di Scienze e Tecnologie Alimentari e Microbiologiche, Facoltà di Agraria, Università degli Studi di Milano, Italy.
Biochem J. 1988 Mar 15;250(3):929-32. doi: 10.1042/bj2500929.
The stereochemical course of the reduction of acetaldehyde to ethanol was investigated by evaluating, with the enzymic system yeast alcohol dehydrogenase/diaphorase and g.c.-m.s., the configuration of [1-2H]ethanol obtained from [1-2H]acetaldehyde with different micro-organisms. Although only S-[1-2H]ethanol was formed, all the micro-organisms showed evidence of the existence of alcohol dehydrogenases with opposite stereospecificity.
通过使用酵母醇脱氢酶/黄递酶酶系统和气相色谱-质谱联用技术，评估从[1-²H]乙醛经不同微生物还原得到的[1-²H]乙醇的构型，研究了乙醛还原为乙醇的立体化学过程。尽管仅形成了S-[1-²H]乙醇，但所有微生物都显示出存在具有相反立体特异性的醇脱氢酶的证据。
