College of Life Science and Technology, Southwest Minzu University, Chengdu, China.
College of Life Science and Technology, Southwest Minzu University, Chengdu, China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Chengdu, China.
Infect Genet Evol. 2021 Aug;92:104879. doi: 10.1016/j.meegid.2021.104879. Epub 2021 Apr 24.
Canine norovirus (CNV) is a diarrhea-causing pathogen in canines. In this study, 268 canine diarrheic fecal samples were collected from 13 pet hospitals across three provinces in China between March 2017 and May 2019, and 7.8% (21/268) samples were detected as CNV-positive by RT-PCR. Phylogenetic analysis of twenty-one CNV RdRp fragments showed that eighteen of the strains clustered in GVI.2, two clustered in GVI.1 and one clustered in GIV.2. The complete RdRp, VP1, and VP2 genes of four GVI.2 strains obtained from three provinces were successfully sequenced. Phylogenetic analyses based on the RdRp, VP1, and VP2 genes showed that the GVI.2 strains from this study were closely related to USA GVI.2 strains. The complete genome of GVI.2 strain Dog/M9/18/CH was successfully sequenced, it was 7905 nucleotides (nt) in length and shared 95.9% nt identity with the sole available, nearly full-length genome of GVI.2 strain genome. To our knowledge, this is first description of the molecular prevalence of CNV in mainland China, and the first report of a complete GVI.2 genome. These findings will extend our understanding of epidemics and the genetic evolution of CNV.
犬诺如病毒（CNV）是一种引起犬类腹泻的病原体。本研究于 2017 年 3 月至 2019 年 5 月期间，从中国三个省的 13 家宠物医院采集了 268 份犬腹泻粪便样本，其中 7.8%（21/268）的样本通过 RT-PCR 检测为 CNV 阳性。对 21 株 CNV RdRp 片段的系统进化分析表明，18 株属于 GVI.2，2 株属于 GVI.1，1 株属于 GIV.2。成功测序了来自三个省份的 GVI.2 株的完整 RdRp、VP1 和 VP2 基因。基于 RdRp、VP1 和 VP2 基因的系统进化分析表明，本研究中的 GVI.2 株与美国 GVI.2 株密切相关。成功测序了 GVI.2 株 Dog/M9/18/CH 的完整基因组，长度为 7905 个核苷酸（nt），与唯一可用的、近乎全长的 GVI.2 株基因组的 nt 同一性为 95.9%。据我们所知，这是首次描述中国大陆地区 CNV 的分子流行情况，也是首次报道 GVI.2 完整基因组。这些发现将扩展我们对 CNV 流行和遗传进化的认识。
