Takano Tomomi, Ryu Mizuki, Doki Tomoyoshi, Kusuhara Hajime
Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada 34-8628, Japan.
Health and Environment Research Institute, Yokkaichi 512-1211, Japan.
Pathogens. 2022 Jun 27;11(7):731. doi: 10.3390/pathogens11070731.
Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for human NoV infection. In the present study, we analyzed the B-cell linear epitope in the P domain of FNoV to confirm the basic immunological features of the FNoV-cat model. B-cell linear epitopes were present in the P2 subdomain. We compared antibody levels to peptides containing the B-cell linear epitope (P-10) in three FNoV-infected cats with time-course changes in viral load and symptom scoring. After FNoV infection, viral shedding and clinical symptoms were shown to improve by elevated levels of antibodies against P-10 in the plasma. This report provides important information for understanding NoV infections in humans and cats.
诺如病毒（NoV）感染仍是全球主要的公共卫生问题。合适的动物模型对于开发有效的诺如病毒疫苗至关重要。我们之前建立了猫诺如病毒（FNoV）-猫模型作为人类诺如病毒感染的替代动物模型。在本研究中，我们分析了FNoV P结构域中的B细胞线性表位，以确认FNoV-猫模型的基本免疫学特征。B细胞线性表位存在于P2亚结构域。我们比较了三只FNoV感染猫中针对含B细胞线性表位（P-10）的肽段的抗体水平与病毒载量和症状评分的时间进程变化。FNoV感染后，血浆中针对P-10的抗体水平升高，病毒排出和临床症状得到改善。本报告为理解人类和猫的诺如病毒感染提供了重要信息。
