Department of Biomedical Engineering, Faculty of Engineering and Architecture, Izmir Katip Celebi University, Izmir, 35620 Turkey.
Department of Biomedical Technologies, Graduate School of Natural and Applied Sciences, Izmir Katip Celebi University, Izmir, 35620 Turkey.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102306. doi: 10.1016/j.pdpdt.2021.102306. Epub 2021 Apr 24.
Photodynamic therapy (PDT) has become an advantageous therapeutic approach for the treatment of select cancers and microbial infections. PDT generates toxic reactive oxygen species as an end product of the interaction between the photosensitizer and light with an appropriate wavelength. Toluidine blue ortho is a photosensitizer that is commonly used in the photodynamic treatment of bacterial infection and a promising photosensitizer for cancer treatment. This study aims to evaluate the potential photo-cytotoxicity of toluidine blue ortho-mediated photodynamic therapy on PC-3 prostate cancer cells.
In this study toluidine blue ortho-mediated photodynamic therapy was assessed on PC-3 cancer cells with various photosensitizer concentrations and light energy densities of the 655-nm diode laser. MTT analysis was used for the determination of the cytotoxicity on the cells and viability/cytotoxicity assay was used for live/dead cell staining after the applications. The mechanism of this application was further analyzed with the determination of intracellular reactive oxygen species and nitric oxide release.
The light applications and the photosensitizer alone did not inhibit the cell viability of PC-3 cells. 20 J/cm laser energy density together with 100 μM photosensitizer concentration resulted in maximum cancer cell death with a rate of approximately 89 %. The level of intracellular reactive oxygen species increased with the increasing parameters of the applications that resulted in more cell death.
This study showed the successful anticancer activity of toluidine blue ortho upon irradiation with 655 nm of laser light against PC-3 cancer cells and it was mediated with the production of reactive oxygen species.
光动力疗法（PDT）已成为治疗某些癌症和微生物感染的有利治疗方法。PDT 产生有毒的活性氧作为光敏剂与适当波长的光相互作用的终产物。甲苯胺蓝邻位是一种常用于光动力治疗细菌感染的光敏剂，也是一种有前途的癌症治疗光敏剂。本研究旨在评估甲苯胺蓝邻位介导的光动力疗法对 PC-3 前列腺癌细胞的潜在光细胞毒性。
在这项研究中，使用不同的光敏剂浓度和 655nm 二极管激光的光能量密度评估了甲苯胺蓝邻位介导的光动力疗法对 PC-3 癌细胞的影响。MTT 分析用于测定细胞的细胞毒性，应用后使用活/死细胞染色进行细胞活力/细胞毒性测定。通过测定细胞内活性氧和一氧化氮的释放，进一步分析了这种应用的机制。
光应用和单独的光敏剂都不会抑制 PC-3 细胞的细胞活力。20 J/cm 激光能量密度与 100 μM 光敏剂浓度相结合，导致癌细胞死亡的最大速率约为 89%。随着应用参数的增加，细胞内活性氧的水平增加，导致更多的细胞死亡。
本研究表明，甲苯胺蓝邻位在接受 655nm 激光照射时对 PC-3 癌细胞具有成功的抗癌活性，并且是通过活性氧的产生介导的。
