Department of neurosurgery, Tumor Hospital Affiliated of Xinjiang Medical University, Xinjiang, China.
Department of Neurosurgery, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
J Neuroimmunol. 2021 Jun 15;355:577575. doi: 10.1016/j.jneuroim.2021.577575. Epub 2021 Apr 20.
Glioblastoma (GBM) immunotherapy, which blocks the checkpoint inhibitor molecule T cell immunoglobulin domain and mucin domain-3 (Tim-3), has potential therapeutic applications. However, not all patients do benefit from the targeted therapy. This study aimed to explore Tim-3 expression correlated chemokine profiles and immune cell infiltration and investigate their potential as prognostic markers of glioblastoma (GBM) immunotherapy. We analyzed transcriptional data of GBM from TCGA database, to measure Tim-3 expression by R package DESeq2 analysis and observed differentially expressed genes in GBM samples with high Tim-3 expression levels. We also probed the relative gene enrichment pathways. Tim-3 expression was evident in biological processes including the recruitment of immune cells. We also identified some chemokines related to Tim-3 expression. The expression levels of CCL18, CXCL13 and CCL7 were significantly higher in GBM tissues with high Tim-3 expression than in GBM tissues with low Tim-3 expression. In addition, exploring the relationship between immune cell infiltration and Tim-3 expression suggested that Tim-3 expression was positively related to significant immune cell infiltration.
胶质母细胞瘤（GBM）免疫疗法通过阻断检查点抑制剂分子 T 细胞免疫球蛋白结构域和粘蛋白结构域 3（Tim-3）来发挥潜在的治疗作用。然而，并非所有患者都能从靶向治疗中获益。本研究旨在探索 Tim-3 表达相关趋化因子谱和免疫细胞浸润，并研究其作为胶质母细胞瘤（GBM）免疫治疗预后标志物的潜力。我们分析了 TCGA 数据库中的 GBM 转录组数据，通过 R 包 DESeq2 分析测量 Tim-3 的表达，并观察高 Tim-3 表达水平的 GBM 样本中差异表达的基因。我们还探讨了相对基因富集途径。Tim-3 表达在包括免疫细胞募集在内的生物学过程中表现明显。我们还确定了一些与 Tim-3 表达相关的趋化因子。在高 Tim-3 表达的 GBM 组织中，CCL18、CXCL13 和 CCL7 的表达水平明显高于低 Tim-3 表达的 GBM 组织。此外，探索免疫细胞浸润与 Tim-3 表达之间的关系表明，Tim-3 表达与显著的免疫细胞浸润呈正相关。
