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评价一种无需 RNA 提取的直接反转录环介导等温扩增法（直接 RT-LAMP）在早期乳腺癌淋巴结转移检测中的应用。

Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction (Direct RT-LAMP) for the Detection of Lymph Node Metastasis in Early Breast Cancer.

机构信息

Division of Hematology/Oncology, Department of Internal Medicine, Daegu Catholic University School of Medicine, Daegu, Korea.

Department of Surgery, Kyungpook National University Chilgok Hospital, School of Medicine, Kyungpook National University, Daegu, Korea.

出版信息

Cancer Res Treat. 2022 Jan;54(1):174-181. doi: 10.4143/crt.2020.749. Epub 2021 Apr 26.

DOI:10.4143/crt.2020.749
PMID:33902166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8756134/
Abstract

PURPOSE

Assessing lymph node metastasis, tumor-derived DNA, or tumor-derived RNA has previously been studied in place of immunohistochemical assay. Because a direct reverse transcription loop-mediated isothermal amplification method (direct RT-LAMP) has been previously developed in order to rapidly identify viruses in place of RNA extraction, our team hypothesized that a direct RT-LAMP assay can be employed as a substitute in order to detect tumor involvement of lymph nodes within breast cancer patients.

MATERIALS AND METHODS

A total amount of 92 lymph nodes removed across 40 patients possessing breast cancer were collected at Kyungpook National University Chilgok Hospital between the months of November 2015 and February 2016. All samples were then evaluated and contrasted via both a direct RT-LAMP assay and routine histopathologic examination.

RESULTS

The sensitivity and specificity of the direct RT-LAMP assay were 85.7% and 100%, respectively. The positive predictive value and negative predictive value were 100% and 94.4%, respectively.

CONCLUSION

Direct RT-LAMP assay is capable of facilitating the detection of sentinel lymph node metastasis within breast cancer patients intraoperatively possessing an excellent sensitivity via a cost-effective and time-saving manner.

摘要

目的

先前已经有研究使用淋巴结转移、肿瘤源性 DNA 或肿瘤源性 RNA 替代免疫组织化学检测来评估。由于先前已经开发出直接逆转录环介导等温扩增方法（direct RT-LAMP）以快速替代 RNA 提取来鉴定病毒，因此我们的团队假设可以使用直接 RT-LAMP 检测来替代检测乳腺癌患者淋巴结中的肿瘤浸润。

材料与方法

2015 年 11 月至 2016 年 2 月期间，我们在庆北国立大学 Chilgok 医院收集了 40 名患有乳腺癌的患者共 92 个淋巴结。所有样本均通过直接 RT-LAMP 检测和常规组织病理学检查进行评估和对比。

结果

直接 RT-LAMP 检测的灵敏度和特异性分别为 85.7%和 100%。阳性预测值和阴性预测值分别为 100%和 94.4%。

结论

直接 RT-LAMP 检测可以通过经济高效和节省时间的方式，在术中对患有乳腺癌的患者进行前哨淋巴结转移的检测，具有出色的灵敏度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/6a22665949fb/crt-2020-749f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/138b85685d4b/crt-2020-749f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/04606b4cdb66/crt-2020-749f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/75cec71647e1/crt-2020-749f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/512e85704247/crt-2020-749f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/6a22665949fb/crt-2020-749f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/138b85685d4b/crt-2020-749f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/04606b4cdb66/crt-2020-749f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/75cec71647e1/crt-2020-749f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/512e85704247/crt-2020-749f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/feff/8756134/6a22665949fb/crt-2020-749f5.jpg

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