12 号心保、心疥爽加和Interceptor Plus 预防大环内酯类药物耐药株（JYD-34）犬心丝虫（犬恶丝虫）的比较疗效。

Comparative preventive efficacy of ProHeart 12, Heartgard Plus and Interceptor Plus against a macrocyclic lactone-resistant strain (JYD-34) of heartworm (Dirofilaria immitis) in dogs.

机构信息

Veterinary Medicine Research and Development, 333 Portage St, Kalamazoo, MI, 49007, USA.

TRS Labs Inc, PO Box 5112, Athens, GA, 30606, USA.

出版信息

Parasit Vectors. 2021 Apr 26;14(1):226. doi: 10.1186/s13071-021-04708-3.

DOI:10.1186/s13071-021-04708-3

PMID:33902689

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8074443/

Abstract

BACKGROUND

The current studies compared ProHeart 12, Heartgard Plus and Interceptor Plus for preventive efficacy against JYD-34, a macrocyclic lactone (ML)-resistant strain of Dirofilaria immitis in dogs.

METHODS

In two studies, each using 24 adult beagles, dogs were allocated to four treatment groups (n = 6): placebo-treated control; ProHeart 12 as per label (0.5 mg/kg moxidectin); Heartgard Plus (HGP) as per label (minimum 6 µg/kg ivermectin); and Interceptor Plus (INP) as per label (minimum 0.5 mg/kg milbemycin oxime). In both studies, ProHeart 12 was administered as a single subcutaneous dose on day 0, and HGP and INP were administered orally on days 0, 30, 60, 90, 120 and 150. In Studies 1 and 2, dogs were inoculated with 50 third-stage heartworm larvae (JYD-34 strain) on days -30 and 165, respectively. In Study 2, treatment for both HGP and INP was continued on days 180, 210, 240, 270, 300 and 330. Adult heartworm recoveries were performed on day 185 in Study 1 and on day 360 in Study 2.

RESULTS

In Studies 1 and 2, all placebo-treated dogs developed adult heartworm infections (geometric mean, 29.9 and 34.9 worms/dog, respectively). A single dose of ProHeart 12 was 100% effective in preventing the development of adult JYD-34 heartworms when treatment was initiated 30 days after heartworm inoculation, while six consecutive monthly doses of HGP and INP were only 10.5% and 14.6% effective, respectively. The mean worm count for the ProHeart 12-treated group was significantly lower (P < 0.0001) than that for the placebo control, HGP- and INP-treated groups. In Study 2, the dogs treated with ProHeart 12 had an efficacy of 98.3%. All dogs treated with HGP and INP for 12 consecutive months had adult heartworms with efficacies of 37.7% and 34.9%, respectively. The mean worm count for the ProHeart 12-treated dogs was significantly lower (P < 0.0001) than those for the control group, HGP- and INP-treated groups.

CONCLUSIONS

A single administration of ProHeart 12 was 98-100% effective in preventing the development of the ML-resistant JYD-34 heartworm strain and was significantly better than multiple consecutive monthly doses of either Heartgard Plus or Interceptor Plus in both studies.

摘要

背景

本研究比较了 ProHeart 12、Heartgard Plus 和Interceptor Plus 三种药物对预防 JYD-34（一种对大环内酯类药物有抗药性的犬心丝虫）的效果。

方法

在两项研究中，各使用 24 只成年比格犬，将狗分为四组（n=6）：安慰剂对照组；按标签（0.5mg/kg 莫昔克丁）使用 ProHeart 12；按标签（最低 6μg/kg 伊维菌素）使用 Heartgard Plus；按标签（最低 0.5mg/kg 米尔贝肟）使用Interceptor Plus。在这两项研究中，ProHeart 12 作为单次皮下剂量在第 0 天给药，Heartgard Plus 和 Interceptor Plus 分别在第 0、30、60、90、120 和 150 天口服给药。在研究 1 和研究 2 中，狗分别在第-30 和第 165 天接种 50 条第三期心丝虫幼虫（JYD-34 株）。在研究 2 中，在第 180、210、240、270、300 和 330 天继续对 Heartgard Plus 和 Interceptor Plus 进行治疗。在研究 1 中，在第 185 天进行成虫心丝虫回收，在研究 2 中在第 360 天进行成虫心丝虫回收。

结果

在研究 1 和研究 2 中，所有安慰剂治疗的狗均发生成虫心丝虫感染（几何均数分别为 29.9 和 34.9 条/狗）。在接种心丝虫后 30 天开始治疗时，单次给予 ProHeart 12 可 100%有效预防 JYD-34 成虫心丝虫的发生，而连续 6 个月给予 Heartgard Plus 和Interceptor Plus 的有效率分别为 10.5%和 14.6%。ProHeart 12 治疗组的平均虫数明显低于（P<0.0001）安慰剂对照组、Heartgard Plus 和 Interceptor Plus 治疗组。在研究 2 中，接受 ProHeart 12 治疗的狗的疗效为 98.3%。连续 12 个月接受 Heartgard Plus 和Interceptor Plus 治疗的所有狗均有心丝虫成虫，疗效分别为 37.7%和 34.9%。ProHeart 12 治疗狗的平均虫数明显低于（P<0.0001）对照组、Heartgard Plus 和 Interceptor Plus 治疗组。