Goyzueta Mamani Luis Daniel, de Carvalho Júlio Cesar, Bonatto Sandro J R, Tanobe Valcineide A O, Soccol Carlos Ricardo
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, P.O. box 19011, Curitiba, Paraná, 81531-990, Brazil.
Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Brazil.
In Vitro Cell Dev Biol Anim. 2021 Apr;57(4):395-403. doi: 10.1007/s11626-021-00560-y. Epub 2021 Apr 26.
This study presents an in vitro evaluation of the antitumor potential of a chitin-like exopolysaccharide (EPS, produced by Mortierella alpina) on Adrenocortical carcinoma cells (ACC) compared to mitotane, a commercial drug commonly used in ACC treatment, and known for its side effects. Techniques of cellular viability determination such as MTT and fluorescence were used to measure the cytotoxic effects of the EPS and mitotane in tumoral cells (H295R) and non-tumoral cells (VERO), observing high cytotoxicity of mitotane and a 10% superior pro-apoptotic effect of the EPS compared to mitotane (p < 0.05). The cytotoxic effect of the EPS was similar to the effect of 50 μM mitotane on tumoral cells (p < 0.05). A decrement of the lysosomal volume was also noted in tumoral cells treated with the EPS. To enhance the antitumor effect, a combination of mitotane at a lower dosage and the EPS (as adjuvant) was also tested, showing a slight improvement of the cytotoxicity effect on tumoral cells. Therefore, the results indicate a cytotoxic effect of the EPS produced by Mortierella alpina on adrenocortical carcinoma, and a possible application in biomedical formulations or additional treatments.
本研究对一种几丁质样胞外多糖（由高山被孢霉产生的EPS）对肾上腺皮质癌细胞（ACC）的抗肿瘤潜力进行了体外评估，并与米托坦进行了比较。米托坦是一种常用于ACC治疗的商业药物，但具有副作用。采用MTT和荧光等细胞活力测定技术来测量EPS和米托坦对肿瘤细胞（H295R）和非肿瘤细胞（VERO）的细胞毒性作用，结果观察到米托坦具有高细胞毒性，且与米托坦相比，EPS的促凋亡作用高10%（p<0.05）。EPS的细胞毒性作用与50μM米托坦对肿瘤细胞的作用相似（p<0.05）。在用EPS处理的肿瘤细胞中还观察到溶酶体体积减小。为了增强抗肿瘤效果，还测试了低剂量米托坦与EPS（作为佐剂）的组合，结果显示对肿瘤细胞的细胞毒性作用略有改善。因此，结果表明高山被孢霉产生的EPS对肾上腺皮质癌具有细胞毒性作用，并可能应用于生物医学制剂或辅助治疗。
