Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Bioresour Technol. 2010 Jul;101(14):5517-22. doi: 10.1016/j.biortech.2010.01.134. Epub 2010 Feb 19.
High-power ultrasound (20 kHz) was applied to modify the physicochemical properties of a high-molecular weight (MW) exopolysaccharide (EPS) from mycelial culture of a medicinal fungus. At 35 W/cm(2) or higher ultrasound power, the apparent and intrinsic viscosities of EPS solution dropped by nearly 85% within 10 min, and the water solubility was increased by more than fourfold. The ultrasonic treatment led to a notable reduction of the maximum MW and a more uniform MW distribution, but no significant change in the primary structure of the EPS molecules. In contrast, the intrinsic viscosity of EPS was reduced by only 20% in 1.0M sulfuric acid at 50 degrees C for 9h. Ultrasound was proven an effective and favorable means for improving the solution properties of high-MW bioactive polysaccharides in mild conditions.
高强超声（20kHz）用于修饰药用真菌菌丝体培养物中高分子量（MW）胞外多糖（EPS）的物理化学性质。在 35W/cm(2)或更高的超声功率下，EPS 溶液的表观和特性黏度在 10 分钟内下降近 85%，溶解度增加了四倍多。超声处理导致 EPS 的最大 MW 显著降低，MW 分布更加均匀，但 EPS 分子的一级结构没有明显变化。相比之下，在 50°C 下 1.0M 硫酸中处理 9 小时，EPS 的特性黏度仅降低了 20%。超声在温和条件下是一种有效且有利的改善高分子量生物活性多糖溶液性质的方法。
