Sydney School of Veterinary Science, The University of Sydney, Camperdown, NSW, Australia.
J Feline Med Surg. 2022 Feb;24(2):116-122. doi: 10.1177/1098612X211009145. Epub 2021 Apr 27.
The aim of this study was to compare the characteristics of fresh and stored feline red blood cells (RBCs) after passage through an 18 μm microaggregate filter.
Nine cats were recruited for a single blood donation using an open collection system. A simulated transfusion using a syringe driver and microaggregate filter was performed over 2 h with half the blood on the day of donation and the other half after 35 days of storage. Differences in haematological parameters, haemolysis percentage and osmotic fragility (OF) were compared on the day of donation pre-filter passage (D0-) vs day of donation post-filter (D0+) or day 35 storage pre-filter (D35-) and post-filter (D35+). Blood was cultured at D0+ and D35+.
There were no statistically significant differences in the D0- vs D0+ comparisons. There were statistically significant ( <0.05) increases in haemolysis percentage, red cell distribution width (RDW) percentage and mean OF, and decreases in packed cell volume (PCV), RBC count, haemoglobin and haematocrit for D0- vs D35-. The same was found for D0- vs D35+ with the addition of a significant increase in mean cell haemoglobin (MCH). For D35- vs D35+ only MCH significantly increased. At day 35, 6/9 units had haemolysis percentages that exceeded 1%. This increased to 8/9 of stored units post-filter passage. All blood units cultured negative.
Fresh RBCs exhibited no in vitro evidence of injury following passage through an 18 μm microaggregate filter. Increased MCH was observed in the stored blood and may represent haemolysis induced by the filter. All other changes can be explained by storage lesion rather than filter passage. The findings highlight the importance of blood banking quality controls and the need for further research to assess the effects of transfusion technique, specifically filter passage, on storage lesion-affected feline blood.
本研究旨在比较通过 18μm 微聚集体过滤器后新鲜和储存猫红细胞（RBC）的特征。
招募 9 只猫进行单次采血，采用开放式采集系统。使用注射器驱动器和微聚集体过滤器模拟输血，在 2 小时内完成，一半血液在采血当天，另一半在储存 35 天后。比较当天过滤前（D0-）与过滤后（D0+）或储存第 35 天过滤前（D35-）与过滤后（D35+）的血液当天的血液学参数、溶血百分比和渗透脆性（OF）的差异。D0+和 D35+时进行血液培养。
D0-与 D0+比较无统计学差异。与 D0-相比，D0-与 D35-时溶血百分比、红细胞分布宽度（RDW）百分比和平均 OF 升高，而红细胞压积（PCV）、RBC 计数、血红蛋白和血细胞比容降低，D0-与 D35-时相同，除了平均细胞血红蛋白（MCH）显著增加。D35-与 D35+时也是如此。在第 35 天，6/9 个单位的溶血百分比超过 1%。过滤后这一比例增加到 8/9。所有血液单位培养均为阴性。
新鲜 RBC 经 18μm 微聚集体过滤器过滤后无体外损伤证据。储存血液中观察到 MCH 增加，可能是过滤器引起的溶血。所有其他变化都可以用储存损伤而不是过滤器通过来解释。这些发现强调了血液银行质量控制的重要性，需要进一步研究评估输血技术（特别是过滤器通过）对储存损伤影响的猫血液的影响。
