Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, Barcelona, Spain.
Animal Blood Bank, Barcelona, Spain.
J Feline Med Surg. 2021 Dec;23(12):1149-1154. doi: 10.1177/1098612X21999990. Epub 2021 Mar 15.
Haemolysis caused by the use of peristaltic infusion pumps (PIPs) has been described in human and canine packed red blood cells (pRBCs). The aim of this study was to evaluate the effects of two different linear PIPs on the haemolysis of feline pRBC units stored for a long time.
Feline pRBC units stored with adenine, dextrose, mannitol and sodium chloride (SAGM) were manufactured. After 35-42 days of storage at 2-4°C, a line administration system with a 180 µm filter was attached to every pRBC bag, the system was drained by gravity alone (8 drops/min) and a 1.3 ml sample was collected (G). A NIKI V4 pump was then used at a flow rate of 25 ml/h, the flow was stopped when the infusion system was filled with blood coming from the infusion pump and another 1.3 ml sample was collected (NK). Finally, an Infusomat FmS pump was evaluated, collecting another 1.3 ml sample (IM). Packed cell volume (PCV) was measured in all samples by microhaematocrit centrifugation, total haemoglobin (HGB) was measured using a specific haemoglobin analyser and, after centrifugation, free HGB was determined by spectrophotometry. The percentage of haemolysis was calculated. Friedman's test was used to compare the samples.
Fifteen feline pRBC units were evaluated. The average degree of haemolysis for sample G (gravity-assisted) was 1.12%. Comparison of the degree of gravity-assisted haemolysis with haemolysis in PIP NK (1.13%) and IM (1.14%) samples revealed no significant differences, with differences of only 0.01% and 0.02%, respectively.
The results of this study demonstrate that the use of two common PIPs in veterinary hospitals does not produce levels of haemolysis that are significantly different than that caused by gravity alone during transfusion of feline pRBCs at a rate of 25 ml/h.
蠕动输注泵（PIP）在人及犬的浓缩红细胞（pRBC）中引起溶血已有相关报道。本研究旨在评估两种不同的线性 PIP 对长时间储存的猫科 pRBC 单位溶血的影响。
制备储存有腺嘌呤、葡萄糖、甘露醇和氯化钠（SAGM）的猫科 pRBC 单位。在 2-4°C 下储存 35-42 天后，每个 pRBC 袋上连接一个带有 180μm 过滤器的管路输注系统，通过重力（8 滴/分钟）排空管路，收集 1.3ml 样本（G）。然后使用 NIKI V4 泵以 25ml/h 的流速输注，当输注系统充满来自输注泵的血液时停止流动，并收集另一个 1.3ml 样本（NK）。最后，评估 Infusomat FmS 泵，收集另一个 1.3ml 样本（IM）。通过微量离心法测量所有样本的红细胞压积（PCV），使用特定的血红蛋白分析仪测量总血红蛋白（HGB），离心后通过分光光度法测定游离 HGB。计算溶血百分比。采用 Friedman 检验比较各组样本。
评估了 15 个猫科 pRBC 单位。G 组（重力辅助）的平均溶血程度为 1.12%。与 PIP NK（1.13%）和 IM（1.14%）样本的溶血程度相比，重力辅助溶血的差异无统计学意义，分别仅相差 0.01%和 0.02%。
本研究结果表明，在以 25ml/h 的速度输注猫科 pRBC 时，与单独重力输注相比，两种常见的兽医医院使用的 PIP 并未产生显著不同的溶血水平。
