Ferreira Rui R F, Graça Rita M C, Cardoso Inês M, Gopegui Rafael R, de Matos Augusto J F
Departments of Veterinary Clinics, Institute for Biomedical Sciences of Abel Salazar, University of Porto, Porto, Portugal.
Animal Blood Bank, Porto, Portugal.
J Vet Emerg Crit Care (San Antonio). 2018 Nov;28(6):512-517. doi: 10.1111/vec.12770. Epub 2018 Oct 9.
Hemolysis is an important quality parameter of packed red blood cells (pRBCs) that is used to assess the cellular integrity of stored blood units. According to human standards, hemolysis at the end of storage must not exceed 1%, as otherwise it may be responsible for decreased transfusion effectiveness and acute life-threatening reactions.
This prospective study was designed to evaluate the hemolysis of canine pRBCs stored in an additive solution containing adenine, dextrose, mannitol, and sodium chloride, and to assess its associations with storage time, duration of the collection process, collection disturbances, and with the final volume and PCV of the pRBCs units.
One hundred eighty pRBCs units were collected from canine donors. Hemolysis of the pRBCs units was determined immediately after processing (t = 0). The units were then stored and retested (t = 1) either before administration (during weeks 2, 3, 4, 5, or 6 of storage) or at the end of the storage period (42 d) if not used.
Mean hemolysis at t = 0 was 0.09% (SD 0.06) and increased during storage, at a more pronounced rate from the 5th (mean values of 0.52%, SD 0.29) to the 6th week (1.2%, SD 0.72). Almost 51% of the units with 36-42 days of shelf-life showed more than 1% hemolysis. Disturbances in the collection process, the volume of the whole blood units, and the volume of stored pRBCs units or their PCV were not related to pRBCs hemolysis.
According to human blood bank recommendations regarding acceptable hemolysis, canine pRBCs stored for more than 35 days should be tested to ensure <1% hemolysis prior to administration.
溶血是浓缩红细胞（pRBCs）的一项重要质量参数，用于评估储存血液单位的细胞完整性。按照人类标准，储存末期的溶血率不得超过1%，否则可能导致输血效果降低以及危及生命的急性反应。
本前瞻性研究旨在评估储存在含有腺嘌呤、葡萄糖、甘露醇和氯化钠的添加剂溶液中的犬pRBCs的溶血情况，并评估其与储存时间、采集过程持续时间、采集干扰以及pRBCs单位的最终体积和红细胞压积的关系。
从犬类供血者采集了180个pRBCs单位。pRBCs单位在处理后立即（t = 0）测定溶血情况。然后将这些单位储存起来，并在给药前（储存第2、3、4、5或6周）或如果未使用则在储存期结束时（42天）重新检测（t = 1）。
t = 0时的平均溶血率为0.09%（标准差0.06），在储存期间有所增加，从第5周（平均值0.52%，标准差0.29）到第6周（1.2%，标准差0.72）增加更为明显。保质期为36 - 42天的单位中，近51%的溶血率超过1%。采集过程中的干扰、全血单位的体积、储存的pRBCs单位的体积或其红细胞压积与pRBCs溶血无关。
根据人类血库关于可接受溶血率的建议，储存超过35天的犬pRBCs在给药前应进行检测，以确保溶血率<1%。
