Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstrasse 19-21, 50935, Cologne, Germany.
German Centre for Infection Research (DZIF), partner site Bonn-Cologne, Cologne, Germany.
Eur J Clin Microbiol Infect Dis. 2021 Nov;40(11):2431-2436. doi: 10.1007/s10096-021-04250-1. Epub 2021 Apr 27.
To establish the optimal detection of third-generation cephalosporin-resistant Enterobacterales (3GCREB), the performance of four different screening methods has been investigated: stool samples without (A) and with (B) pre-enrichment and rectal swabs without (C) and with (D) pre-enrichment were contrasted. Pre-enrichment approaches (B and D) increased the detection of 3GCREB carriers by 29.4% (20/68 3GCREB carriers only found using pre-enrichment, p < 0.0001) compared to direct plating approaches (A and C). Moreover, the study demonstrates a minor advantage of stool samples in contrast to rectal swabs in both cases (with and without pre-enrichment). Registration number: DRKS00022520, 24 July 2020.
为了确定最佳的第三代头孢菌素耐药肠杆菌科（3GCREB）检测方法，本研究对四种不同的筛选方法进行了评估：未进行（A）和进行（B）预增菌的粪便样本，以及未进行（C）和进行（D）预增菌的直肠拭子。预增菌方法（B 和 D）比直接平板培养方法（A 和 C）增加了 29.4%（仅在预增菌时发现的 3GCREB 携带者 20/68，p<0.0001）对 3GCREB 携带者的检测。此外，本研究还表明，在进行和不进行预增菌的情况下，粪便样本在两种情况下（有和没有预增菌）均比直肠拭子具有轻微优势。注册号：DRKS00022520，2020 年 7 月 24 日。
