新生儿坏死性小肠结肠炎相关的 DNA 甲基化特征在受累个体的粪便样本中是明显的。
Neonatal necrotizing enterocolitis-associated DNA methylation signatures in the colon are evident in stool samples of affected individuals.
机构信息
Department of Pediatrics, Division of Newborn Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
Departments of Obstetrics, Gynecology & Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
出版信息
Epigenomics. 2021 Jun;13(11):829-844. doi: 10.2217/epi-2021-0078. Epub 2021 Apr 27.
Abstract
Neonatal necrotizing enterocolitis (NEC) is a deadly and unpredictable gastrointestinal disease, for which no biomarker exists. We aimed to describe the methylation patterns in stool and colon from infants with NEC. We performed a high-resolution genome-wide epigenomic analysis using solution-phase hybridization and next-generation sequencing of bisulfite-converted DNA. Our data reveal significant genomic hypermethylation in NEC tissues compared with non-NEC controls. These changes were more pronounced in regions outside CpG islands and gene regulatory elements, suggesting that NEC-specific hypermethylation is not a nonspecific global phenomenon. This study provides evidence of a methylomic signature associated with NEC that is detectable noninvasively and provides a new opportunity for the development of a novel diagnostic method for NEC.
摘要
新生儿坏死性小肠结肠炎（NEC）是一种致命且难以预测的胃肠道疾病，目前尚无生物标志物。我们旨在描述患有 NEC 的婴儿粪便和结肠中的甲基化模式。我们使用溶液相杂交和亚硫酸氢盐转化 DNA 的下一代测序进行了高分辨率全基因组表观基因组分析。与非 NEC 对照相比，我们的数据显示 NEC 组织中存在显着的基因组高甲基化。这些变化在 CpG 岛和基因调控元件之外的区域更为明显，这表明 NEC 特异性高甲基化不是非特异性的全局现象。这项研究提供了与 NEC 相关的甲基组特征的证据，该特征可通过非侵入性检测到，并为开发 NEC 的新型诊断方法提供了新的机会。
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