Department oF Pathology, Theodor Bilharz Research Institute, Giza, Egypt.
Faculty of Biotechnology, October University for Modern Sciences and Arts, Giza, Egypt.
Asian Pac J Cancer Prev. 2021 Apr 1;22(4):1105-1113. doi: 10.31557/APJCP.2021.22.4.1105.
To assess the expression of IL-4, IL-17 and CD-163 as well as study of IL6-572 C/G gene polymorphism in chronic HCV and HCC on top of HCV.
Sixty HCC specimens and 60 adjacent hepatic tissue with HCV of different grades of necro-inflammation and different stages of fibrosis. In addition to 55 HCV, 60 HCC and 50 healthy venous blood samples for evaluation of IL6-572 C/G gene polymorphism.
high expression of IL-4, IL-17 and CD163 in higher grades of activity, late stages of fibrosis and higher degrees of steatosis of HCV. IL-4 and CD163 showed higher expression in advanced grades of HCC, while IL-17 more expressed in lower grades. No significant difference in IL6-572 C/G gene polymorphism among studied groups regarding G/C, G/G, C/C frequencies or G and C allele's frequencies.
IL-4, IL-17 and CD163 were associated with HCV severity. Their expression in HCC suggests their important role in HCC development. Blocking of these proteins may be a good target to control inflammation in HCV and can hinder progression to cirrhosis then to HCC. On the other hand, IL6-572 promoter gene polymorphism is neither associated with HCV infection nor with HCC development and its progression.
.
评估慢性 HCV 和 HCC 中 IL-4、IL-17 和 CD-163 的表达，并研究 IL6-572 C/G 基因多态性。
对 60 例 HCC 标本和 60 例伴有不同坏死炎症程度和不同纤维化阶段的 HCV 的肝组织进行研究。除了 55 例 HCV 外，还评估了 60 例 HCC 和 50 例健康静脉血样本的 IL6-572 C/G 基因多态性。
IL-4、IL-17 和 CD163 在 HCV 活性较高、纤维化晚期和脂肪变性程度较高的患者中高表达。IL-4 和 CD163 在 HCC 的晚期分级中表达较高，而 IL-17 在较低的分级中表达较高。在研究组中，IL6-572 C/G 基因多态性在 G/C、G/G、C/C 频率或 G 和 C 等位基因频率方面没有显著差异。
IL-4、IL-17 和 CD163 与 HCV 的严重程度有关。它们在 HCC 中的表达表明它们在 HCC 发展中起着重要作用。阻断这些蛋白可能是控制 HCV 炎症的一个很好的靶点，并可以阻止其进展为肝硬化，然后进展为 HCC。另一方面，IL6-572 启动子基因多态性既与 HCV 感染无关，也与 HCC 的发展及其进展无关。
