Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Science, Daneshgah Street, Tabriz, Iran.
Tuberculosis and Lung Disease Research Center & Rahat Breathe and Sleep Resaerch, Center, Tabriz University of Medical Science, Tabriz, Iran.
Asian Pac J Cancer Prev. 2021 Apr 1;22(4):1157-1163. doi: 10.31557/APJCP.2021.22.4.1157.
After cardiovascular illness, cancer is the one of the main and second cause of death in the worldwide. Despite significant advances in this field, low survival, drug resistance, and side effects of chemotherapy remain an unsolved problem. Due to the high mortality rate among cancer patients, finding the new substance to treatment with low side effects is important. Previous studies have been informed that positive effects of herbal medicines on cancer patients, which are very efficient in the treatment of cancer.
In this study, the antitumor effect of ethanolic Terminalia catappa leaf extract (TCE) on MCF-7, MDA-231, and A549 cell lines was examined. For this reason, the effects of TCE on cell migration, gene expression, and growth were investigated by scratch, test, real-time PCR (qPCR) qPCR, and MTT tests respectively.
As a reported by the MTT outcomes, TCE significantly decreased the viability of A549, MCF-7, and MDA-231 cells (P < 0.05). Moreover, genes expression patterns that are related to proliferation (miR-21, miR-34a), migration (MMP-13, Vimentin), and apoptosis (Cas-3, Cas-8, Cas-9, Bcl-2, Bax) also have changed significantly after treatment with TCE. Also, in the A549 cell line, Bax (p value: 0.029), Cas-9 (p value: 0.00023), miR-34a (p value: 0.031), Bcl-2 (p value: 0.0076), MMP-13 (p value: 0.041), Cas-3 (p value: 0.00051) and in MCF-7 cell line Bax (p value: 0.0004), Cas-3 (p value: 0.0003), Cas-9(p value: 0.037), miR-34a (p value: 0.005), Bcl-2(pvalue:0.0007), mir-21(p value:0.016), MMP-13(p value: 0.011) and in MDA-231 cell line Bax(p value<0.0001), Cas-3(p value: 0.003), Cas-9(p value: 0.0004). mir-34a (p value:0.0019), Bcl-2(p value:0.0023), MMP-13(p value: 0.032) have significantly changed compare to control group.
The outcomes of this research determined that T. Catappa might be a potential source of antitumor compounds and could be a candidate for further research.
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心血管疾病后，癌症是全球主要的和第二大死亡原因之一。尽管在这一领域取得了重大进展，但生存率低、耐药性和化疗副作用仍然是一个未解决的问题。由于癌症患者的死亡率高，寻找副作用低的新物质进行治疗非常重要。先前的研究表明，草药对癌症患者有积极的影响，在癌症治疗中非常有效。
在这项研究中，考察了乙醇诃子叶提取物（TCE）对 MCF-7、MDA-231 和 A549 细胞系的抗肿瘤作用。为此，分别通过划痕试验、测试、实时 PCR（qPCR）和 MTT 试验研究了 TCE 对细胞迁移、基因表达和生长的影响。
正如 MTT 结果所报告的，TCE 显著降低了 A549、MCF-7 和 MDA-231 细胞的活力（P<0.05）。此外，与增殖（miR-21、miR-34a）、迁移（MMP-13、Vimentin）和凋亡（Cas-3、Cas-8、Cas-9、Bcl-2、Bax）相关的基因表达模式也在 TCE 治疗后发生了显著变化。此外，在 A549 细胞系中，Bax（p 值：0.029）、Cas-9（p 值：0.00023）、miR-34a（p 值：0.031）、Bcl-2（p 值：0.0076）、MMP-13（p 值：0.041）、Cas-3（p 值：0.00051）和 MCF-7 细胞系中，Bax（p 值：0.0004）、Cas-3（p 值：0.0003）、Cas-9（p 值：0.037）、miR-34a（p 值：0.005）、Bcl-2（p 值：0.0007）、miR-21（p 值：0.016）、MMP-13（p 值：0.011）和 MDA-231 细胞系中，Bax（p 值<0.0001）、Cas-3（p 值：0.003）、Cas-9（p 值：0.0004）。miR-34a（p 值：0.0019）、Bcl-2（p 值：0.0023）、MMP-13（p 值：0.032）与对照组相比有显著变化。
本研究结果表明，诃子可能是抗肿瘤化合物的潜在来源，可作为进一步研究的候选药物。
