Shanehbandi Dariush, Zarredar Habib, Asadi Milad, Zafari Venus, Esmaeili Shiva, Seyedrezazadeh Ensiyeh, Soleimani Zahra, Sabagh Jadid Hamed, Eyvazi Shirin, Feyziniya Sara, Moghadam Sedigheh Bamdad, Khalili Majid
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Science, Daneshgah Street, Tabriz, Iran.
J Gastrointest Cancer. 2021 Mar;52(1):99-105. doi: 10.1007/s12029-019-00349-z.
Colorectal cancer (CRC) is one of the most lethal and prevalent cancers throughout the world. Despite the remarkable advance in the field, drug resistance still remains as an unresolved problem in cancer. Hence, finding effective compounds with minimal side effects to fight cancer is of central priority. Herbal products have been traditionally used to prevent and treat a variety of diseases.
In the present study, the antitumor effect of Terminalia catappa plant ethanolic extract (TCE) was assessed on SW480 CRC model cell line. In this regard, effects of TCE were evaluated on the proliferation, apoptosis, and migration of SW480 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Annexin V/PI flow cytometry, and scratch tests, respectively. Furthermore, changes in the expression of genes involved in these events including Bax, Bcl-2, Caspase 3, Caspase 8, Caspase 9, MMP-13, miR-21, and miR-34a were measured by quantitative real-time PCR (qRT-PCR).
According to the MTT results, TCE reduced the proliferation of SW480 cells significantly. The flow cytometry test also revealed a notable rate of apoptosis induction after TCE treatment. An inhibitory effect on cell migration was also evident in scratch test. Expression patterns of the assessed genes also changed subsequent to TCE treatment.
The results of this study indicated that T. catappa could be considered as a potential source of anticancer compounds and a candidate for further investigations.
结直肠癌（CRC）是全球最致命且最常见的癌症之一。尽管该领域取得了显著进展，但耐药性仍是癌症中一个尚未解决的问题。因此，寻找副作用最小的有效抗癌化合物是重中之重。草药产品传统上一直用于预防和治疗多种疾病。
在本研究中，对榄仁树植物乙醇提取物（TCE）在SW480结直肠癌模型细胞系上的抗肿瘤作用进行了评估。在这方面，分别通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法、膜联蛋白V/碘化丙啶流式细胞术和划痕试验评估TCE对SW480细胞增殖、凋亡和迁移的影响。此外，通过定量实时聚合酶链反应（qRT-PCR）测量参与这些事件的基因包括Bax、Bcl-2、半胱天冬酶3、半胱天冬酶8、半胱天冬酶9、基质金属蛋白酶-13、miR-21和miR-34a的表达变化。
根据MTT结果，TCE显著降低了SW480细胞的增殖。流式细胞术检测还显示TCE处理后凋亡诱导率显著。划痕试验中对细胞迁移的抑制作用也很明显。TCE处理后评估基因的表达模式也发生了变化。
本研究结果表明，榄仁树可被视为抗癌化合物的潜在来源和进一步研究的候选对象。
