Hessenauer Maximilian, Vaghela Ravikumar, Körner Carolin, von Hörsten Stephan, Pobel Christoph, Gage Daniel, Müller Claudia, Salehi Sahar, Horch Raymund E, Arkudas Andreas
Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany.
Department of Materials Science and Engineering for Metals, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany.
Tissue Eng Part C Methods. 2021 Jun;27(6):357-365. doi: 10.1089/ten.TEC.2021.0024.
in reconstructive surgery seeks to generate bioartificial tissue substitutes. The arteriovenous (AV) loop allows the generation of axially vascularized tissue constructs. Cellular mechanisms of this vascularization process are largely unclear. In this study, we developed two different chamber models for intravital microscopy and imaging of the AV loop in the rat. Multiple design variations were implanted and the stability of the chamber and AV loop patency was tested . Our novel chamber facilitates repetitive observation of the AV loop using fluorescence-enhanced intravital microscopy. This technique can be used for daily evaluation of leukocyte-endothelial cell interactions, vascularization, and tissue formation in the AV loop model on 14 consecutive days. Therefore, our newly developed model for intravital microscopy will provide better understanding of cellular and molecular processes in in the AV loop. Moreover, it supports initiation of the novel approaches for therapeutic applications. Impact statement In the Arteriovenous (AV) loop, axially vascularized tissue can be generated and modified using different approaches. Cellular mechanisms of this vascularization process are largely unclear. We managed to develop an intravital microscopy model for long-term observation of intravascular and perivascular events in the AV loop. Leukocyte-endothelial cell interactions, vascularization, and tissue formation in the AV loop can now be evaluated on a day-to-day basis. This will provide better understanding of cellular and molecular processes happening during within the AV loop.
在重建手术中，旨在生成生物人工组织替代物。动静脉（AV）环可用于生成轴向血管化的组织构建体。该血管化过程的细胞机制在很大程度上尚不清楚。在本研究中，我们开发了两种不同的腔室模型，用于对大鼠的AV环进行活体显微镜检查和成像。植入了多种设计变体，并测试了腔室的稳定性和AV环的通畅性。我们的新型腔室便于使用荧光增强活体显微镜对AV环进行重复观察。该技术可用于连续14天每天评估AV环模型中的白细胞-内皮细胞相互作用、血管化和组织形成。因此，我们新开发的活体显微镜模型将有助于更好地理解AV环中的细胞和分子过程。此外，它支持启动治疗应用的新方法。影响声明：在动静脉（AV）环中，可以使用不同方法生成和修改轴向血管化组织。该血管化过程的细胞机制在很大程度上尚不清楚。我们成功开发了一种活体显微镜模型，用于长期观察AV环中的血管内和血管周围事件。现在可以每天评估AV环中的白细胞-内皮细胞相互作用、血管化和组织形成。这将有助于更好地理解AV环内发生的细胞和分子过程。
