Gao Zhenya, Yu Fang, Jia Huanxia, Ye Zhuo, Yao Shijie
School of Medicine, Xuchang University, Xuchang, China.
The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
J Int Med Res. 2021 Apr;49(4):3000605211008325. doi: 10.1177/03000605211008325.
To detect the expression of FK506-binding protein 5 (FKBP5) in human papillary thyroid carcinoma (PTC) tissues, and explore its possible role in the progression of PTC.
FKBP5 expression levels were assessed in 115 PTC tissues and corresponding normal tissues by immunohistochemistry. We also examined the correlations between FKBP5 expression and clinicopathological factors and survival in 75 patients with PTC. The effects of FKBP5 on the proliferation and apoptosis of PTC cells were detected by colony-formation, MTT, and flow cytometry assays, respectively. We further investigated the effects of FKBP5 on tumor growth in mice.
We revealed high expression levels of FKBP5 in human PTC tissues compared with normal tissues. Furthermore, high FKBP5 expression was associated with an increased incidence of intraglandular dissemination, and lower overall and progression-free survival. FKBP5 depletion remarkably suppressed the proliferation and induced apoptosis of PTC cells . FKBP5 further contributed to the growth of PTC tumors in mice.
The results of this study demonstrated the potential involvement of FKBP5 in the progression of PTC, and confirmed FKBP5 as a novel therapeutic target for PTC treatment.
检测FK506结合蛋白5（FKBP5）在人甲状腺乳头状癌（PTC）组织中的表达，并探讨其在PTC进展中的可能作用。
采用免疫组织化学法评估115例PTC组织及相应正常组织中FKBP5的表达水平。我们还检测了75例PTC患者中FKBP5表达与临床病理因素及生存情况之间的相关性。分别通过集落形成、MTT和流式细胞术检测FKBP5对PTC细胞增殖和凋亡的影响。我们进一步研究了FKBP5对小鼠肿瘤生长的影响。
与正常组织相比，我们发现人PTC组织中FKBP5表达水平较高。此外，FKBP5高表达与腺内播散发生率增加、总生存期和无进展生存期降低相关。FKBP5缺失显著抑制PTC细胞的增殖并诱导其凋亡。FKBP5进一步促进了小鼠PTC肿瘤的生长。
本研究结果表明FKBP5可能参与PTC的进展，并证实FKBP5是PTC治疗的一个新的治疗靶点。
