厄瓜多尔手工软奶酪中单核细胞增生李斯特菌的检测与基因分型
Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador.
作者信息
Espinosa-Mata Estefania, Mejía Lorena, Villacís José E, Alban Viviana, Zapata Sonia
机构信息
Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador.
Centro de Referencia Nacional de Resistencia a los Antimicrobianos Instituto Nacional de Investigación en Salud Pública "Leopoldo Izquieta Pérez", Quito, Ecuador; Carrera de Bioquímica Clínica, Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador.
出版信息
Rev Argent Microbiol. 2022 Jan-Mar;54(1):53-56. doi: 10.1016/j.ram.2021.02.013. Epub 2021 Apr 24.
Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an important L. monocytogenes vehicle. In Ecuador, soft cheese is consumed in 84.3% of urban households. We determined the prevalence of L. monocytogenes and serogroups in 260 fresh artisanal soft cheese samples collected in 18 of 24 Ecuadorian provinces. Listeria spp. detection was carried out by culture-dependent and independent methods; 14.23% of samples were positive for L. monocytogenes. Serogroup IVb was found in 83.78% of the food isolates. Serogroups IIb and IIa were present in 8.11% of our isolates. To our knowledge, this is the first report of L. monocytogenes serogroups associated with food in Ecuador; we also found serogroup similarities between cheese isolates and clinical isolates.
单核细胞增生李斯特菌是与食源性疾病相关的最重要细菌之一，软奶酪是单核细胞增生李斯特菌的重要传播载体。在厄瓜多尔，84.3%的城市家庭消费软奶酪。我们测定了从厄瓜多尔24个省份中的18个省份采集的260份新鲜手工软奶酪样本中单核细胞增生李斯特菌及其血清群的流行情况。采用依赖培养和不依赖培养的方法检测李斯特菌属；14.23%的样本单核细胞增生李斯特菌呈阳性。在83.78%的食品分离株中发现了IVb血清群。在我们分离的菌株中，IIb和IIa血清群占8.11%。据我们所知，这是厄瓜多尔首次报道与食品相关的单核细胞增生李斯特菌血清群；我们还发现奶酪分离株与临床分离株之间存在血清群相似性。
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