毒蕈碱受体介导的人神经母细胞瘤（SH-SY5Y）细胞中磷脂酰肌醇的水解对百日咳毒素敏感。

Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin.

作者信息

Mei L, Yamamura H I, Roeske W R

机构信息

Department of Pharmacology, University of Arizona, Tucson 85724.

出版信息

Brain Res. 1988 May 3;447(2):360-3. doi: 10.1016/0006-8993(88)91140-7.

DOI:10.1016/0006-8993(88)91140-7

PMID:3390705

Abstract

Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/10(6) cells to 42.4 fmol/10(6) cells (P less than 0.05) and 22.1 fmol/10(6) cells (P less than 0.01) in the absence and presence of 1 microgram/ml and 10 micrograms/ml pertussis toxin, respectively while the EC50 values did not change. Cholera toxin (1 mg/ml) did not alter carbachol-stimulated [3H]IP1 accumulation in these cells. These results suggest that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.

摘要

利用人神经母细胞瘤细胞系（SH-SY5Y）研究了百日咳毒素或霍乱毒素对卡巴胆碱刺激的肌醇-1-磷酸（[3H]IP1）积累的影响。在不存在和存在1微克/毫升及10微克/毫升百日咳毒素的情况下，SH-SY5Y细胞中卡巴胆碱刺激的[3H]IP1积累最大值分别从51.4飞摩尔/10^6个细胞降至42.4飞摩尔/10^6个细胞（P<0.05）和22.1飞摩尔/10^6个细胞（P<0.01），而半数有效浓度（EC50）值未改变。霍乱毒素（1毫克/毫升）未改变这些细胞中卡巴胆碱刺激的[3H]IP1积累。这些结果表明，一种对百日咳毒素敏感的G蛋白可能参与了SH-SY5Y细胞中毒蕈碱受体-磷脂酰肌醇水解偶联过程。