Wang Hui, Hu Xue, Li Minjing, Pan Zhaohai, Li Defang, Zheng Qiusheng
Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, Xinjiang 832003, P.R. China.
Yantai Key Laboratory of Pharmacology of Traditional Chinese Medicine in Tumor Metabolism, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
Oncol Lett. 2021 Jun;21(6):453. doi: 10.3892/ol.2021.12714. Epub 2021 Apr 8.
Research suggests that daphnoretin exhibits a diverse array of antitumor mechanisms and pharmacological activities. However, there is no definitive explanation for the antitumor mechanisms of daphnoretin in malignant melanoma. In the present study, MTT and colony formation assays demonstrated that daphnoretin significantly inhibited the proliferation of melanoma A375 and B16 cells. Following treatment with daphnoretin, apoptotic bodies were observed in A375 and B16 cells via Hoechst 33258 staining. Furthermore, western blot analysis revealed that the apoptosis-related proteins cleaved caspase-3, cleaved caspase-9, Bax, cytochrome and apoptotic protease-activating factor 1 were significantly upregulated, while the expression levels of caspase-3, caspase-9 and Bcl-2 were downregulated in A375 and B16 cells. Flow cytometry and fluorescence microscopy revealed that daphnoretin induced higher levels of reactive oxygen species (ROS). Therefore, the results of the present study indicated that daphnoretin induced ROS-mediated mitochondria apoptosis in human (A375) and murine (B16) malignant melanoma cells.
研究表明,瑞香素具有多种抗肿瘤机制和药理活性。然而,瑞香素在恶性黑色素瘤中的抗肿瘤机制尚无确切解释。在本研究中,MTT和集落形成试验表明,瑞香素显著抑制黑色素瘤A375和B16细胞的增殖。用瑞香素处理后,通过Hoechst 33258染色在A375和B16细胞中观察到凋亡小体。此外,蛋白质印迹分析显示,凋亡相关蛋白裂解的半胱天冬酶-3、裂解的半胱天冬酶-9、Bax、细胞色素和凋亡蛋白酶激活因子1在A375和B16细胞中显著上调,而半胱天冬酶-3、半胱天冬酶-9和Bcl-2的表达水平下调。流式细胞术和荧光显微镜检查显示,瑞香素诱导更高水平的活性氧(ROS)。因此,本研究结果表明,瑞香素诱导人(A375)和小鼠(B16)恶性黑色素瘤细胞中ROS介导的线粒体凋亡。