[缺氧诱导因子-1α在小鼠下颌第一磨牙萌出过程中的表达及其意义]
[Expression of hypoxia inducible factor-1alpha during mouse mandibular first molar eruption and its significance].
作者信息
Hu Xiu, Kang Fei-Wu
机构信息
Department of Oral and Maxillofacial Surgery, School ＆ Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration. Shanghai 200072, China. E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2021 Feb;30(1):13-16.
PURPOSE
To investigate the expression of hypoxia inducible factor-1alpha (HIF-1α) during the eruption of mouse lower first molar, and to explore the correlation between its expression and the activity of osteoclasts.
METHODS
Mouse mandibles were dissected from mice at day 1.5 to 14.5 after birth. They were then serially sectioned and stained respectively using hemotoxylin, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemical staining. SPSS 25.0 software package was used for data analysis.
RESULTS
During the eruption of mouse lower first molar, the thickness of the crown alveolar bone gradually decreased, so did the number of osteoclasts and the expression of HIF-1α.
CONCLUSIONS
During the eruption of mouse lower first molar, the less expression of HIF-1α, the fewer osteoclasts there were. HIF-1α may be involved in the eruption of mouse lower first molar by regulating the activity of osteoclasts.
目的
研究缺氧诱导因子-1α（HIF-1α）在小鼠下颌第一磨牙萌出过程中的表达情况，并探讨其表达与破骨细胞活性之间的相关性。
方法
取出生后1.5至14.5天小鼠的下颌骨，进行连续切片，分别采用苏木精染色、抗酒石酸酸性磷酸酶（TRAP）染色及免疫组织化学染色。使用SPSS 25.0软件包进行数据分析。
结果
在小鼠下颌第一磨牙萌出过程中，冠周牙槽骨厚度逐渐减小，破骨细胞数量及HIF-1α表达也逐渐降低。
结论
在小鼠下颌第一磨牙萌出过程中，HIF-1α表达越少，破骨细胞数量越少。HIF-1α可能通过调节破骨细胞活性参与小鼠下颌第一磨牙的萌出过程。
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