Qin Han, Cai Jun
Department of Stomatology, The Lianyungang Affiliated Hospital of Xuzhou Medical University, #182 Tongguan Road, Lianyungang 222002, Jiangsu Province, China.
Department of Anesthesia, The Maternal and Child Health Hospital of Lianyungang City, Lianyungang 222006, Jiangsu Province, China.
Open Life Sci. 2023 Aug 8;18(1):20220663. doi: 10.1515/biol-2022-0663. eCollection 2023.
This study aimed to investigate the effect of periostin (PN) on the expression of receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), microtubule-associated protein1 light chain 3B (LC3B), and Beclin1 in mouse alveolar bone specimens and cultured osteoblasts , to preliminarily explore the role of PN and autophagy in remodeling bone metabolism during tooth eruption. Mice at 5 days of age were injected with 75 ng/mL recombinant PN protein under the periosteum for 3 consecutive days according to the standard of 1 mL/100 g/day. Then, their mandibles were removed, and the expression of bone metabolic and autophagy factors was detected by immunohistochemistry. Mouse osteoblast-like cells cultured were treated with recombinant PN at a concentration of 75 ng/mL. The changes in the aforementioned indicators were compared again by immunofluorescence and western blotting 72 h after dosing. The results of the mouse samples showed that the protein expression of RANKL, LC3B, and Beclin1 decreased, accompanied by the decrease in RANKL/OPG ratio. However, OPG protein expression increased in the dosing group. Immunofluorescence and western blotting results of osteoblasts cultured showed that the protein expression of RANKL, LC3B, Beclin1, and the RANKL/OPG ratio in the experimental group decreased, but OPG expression increased. PN may regulate alveolar bone metabolism during tooth eruption by inhibiting the RANKL/OPG ratio and autophagy, which will provide a new research perspective for further exploration of the mechanisms during tooth eruption.
本研究旨在探讨骨膜蛋白（PN）对小鼠牙槽骨标本及培养的成骨细胞中核因子κB受体激活剂配体（RANKL）、骨保护素（OPG）、微管相关蛋白1轻链3B（LC3B）和Beclin1表达的影响，初步探究PN和自噬在牙齿萌出过程中骨代谢重塑中的作用。5日龄小鼠按照1 mL/100 g/天的标准，在骨膜下连续3天注射75 ng/mL重组PN蛋白。然后，取下它们的下颌骨，通过免疫组织化学检测骨代谢和自噬因子的表达。将培养的小鼠成骨样细胞用浓度为75 ng/mL的重组PN处理。给药72小时后，通过免疫荧光和蛋白质印迹法再次比较上述指标的变化。小鼠样本结果显示，RANKL、LC3B和Beclin1的蛋白表达下降，同时RANKL/OPG比值降低。然而，给药组中OPG蛋白表达增加。培养的成骨细胞的免疫荧光和蛋白质印迹结果显示，实验组中RANKL、LC3B、Beclin1的蛋白表达及RANKL/OPG比值降低，但OPG表达增加。PN可能通过抑制RANKL/OPG比值和自噬来调节牙齿萌出过程中的牙槽骨代谢，这将为进一步探索牙齿萌出机制提供新的研究视角。
