CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration, Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, PO Box 416, Chengdu, Sichuan, 610041, China.
University of Chinese Academy of Science, Beijing, China.
Microsc Res Tech. 2021 Oct;84(10):2337-2350. doi: 10.1002/jemt.23788. Epub 2021 Apr 28.
In this research, the fruits and seeds' surface morphological features and their taxonomic significance were determined. Additionally, useful traits for delimitation of taxa of Rosa sericea complex were studied. In this research, three taxa of the complex R. sericea, R. omeiensis, and R. sikangensis have been studied. A total of 41 populations and 130 specimens of seeds, and 35 populations and 168 specimens of fruits from 10 different geographical regions of southwest China have been examined. The surface micro-morphological characters of the seeds have been studied through scanning electron microscopy to distinguish important characteristics of the complex taxa. Forty-four different qualitative and quantitative characteristics were observed. Principal component analysis was used to simplify and elucidate the relationship between the studied characters of different population based on the morphology of fruits and seeds' features. Moreover, the numerical taxonomic characters and their variation with elevation have been studied in these species. The most interesting characters observed on the surface of the seeds were stomata, and trichomes, for which density was more at the dorsal and ventral parts, while rarely present on the lateral parts. The fruits and seeds' surface morphology of the studied species was complicated and some variation in the characters, that is, persistent calyx shape, the shape of fruits and seeds, stomata shape, and trichomes size were found significant for delimitation of taxa in the complex. The purpose of this study is to identify the taxa of R. sericea complex based on fruits and seeds' morphological characters, and identification keys.
在这项研究中，确定了果实和种子的表面形态特征及其分类学意义。此外，还研究了用于界定蔷薇属复杂种的有用特征。在这项研究中，研究了该复合体中的三个种，即绢毛蔷薇、峨眉蔷薇和滇刺枣。总共检查了来自中国西南地区 10 个不同地理区域的 41 个种群和 130 个种子标本，以及 35 个种群和 168 个果实标本。通过扫描电子显微镜研究了种子的表面微观形态特征，以区分复杂种的重要特征。观察到了 44 种不同的定性和定量特征。主成分分析用于简化和阐明基于果实和种子特征的不同种群之间研究特征之间的关系。此外，还研究了这些物种中数值分类特征及其随海拔的变化。在种子表面观察到的最有趣的特征是气孔和毛状体，其密度在背部和腹部较高，而在侧面很少。所研究物种的果实和种子表面形态复杂，某些特征存在变化，即宿存花萼形状、果实和种子形状、气孔形状和毛状体大小对于该复合体中种的界定具有重要意义。本研究的目的是根据果实和种子的形态特征以及鉴定钥匙来识别蔷薇属复杂种的分类。
