Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
Department of Animal Production, Federal University of Technology Minna, PMB 65, Minna, Niger, Nigeria.
J Vet Sci. 2021 May;22(3):e29. doi: 10.4142/jvs.2021.22.e29. Epub 2021 Mar 5.
West Nile virus (WNV), a neurotropic arbovirus, has been detected in mosquitos, birds, wildlife, horses, and humans in Malaysia, but limited information is available on WNV infection in Malaysian pigs. We tested 80 archived swine serum samples for the presence of WNV antibody and West Nile (WN) viral RNA using ID Screen West Nile Competition Multi-species enzyme-linked immunosorbent assay kits and WNV-specific primers in reverse transcription polymerase chain reaction assays, respectively. A WNV seroprevalence of 62.5% (50/80) at 95% confidence interval (51.6%-72.3%) was recorded, with a significantly higher seroprevalence among young pigs (weaner and grower) and pigs from south Malaysia. One sample was positive for Japanese encephalitis virus antibodies; WN viral RNA was not detected in any of the serum samples.
西尼罗河病毒（WNV）是一种嗜神经的虫媒病毒，已在马来西亚的蚊子、鸟类、野生动物、马和人类中检测到，但关于马来西亚猪的 WNV 感染的信息有限。我们使用 ID Screen West Nile 竞争多物种酶联免疫吸附测定试剂盒和 WNV 特异性引物在逆转录聚合酶链反应检测中，分别检测了 80 份存档猪血清样本中是否存在 WNV 抗体和西尼罗河（WN）病毒 RNA。记录到 WNV 血清阳性率为 62.5%（50/80），置信区间为 95%（51.6%-72.3%），其中仔猪（育肥猪和生长猪）和来自马来西亚南部的猪的血清阳性率明显更高。一份样本对日本脑炎病毒抗体呈阳性；WN 病毒 RNA 未在任何血清样本中检测到。
