Gambhir K K, Parui R, Nerurkar S G, Dave N, Mathews J, Mehrotra P P, Curry C L
Department of Medicine, Howard University, Washington, D.C. 20059.
Clin Biochem. 1988 Jun;21(3):163-5. doi: 10.1016/0009-9120(88)90004-5.
We report a modification of the technique of Mahoney et al. (Blood 1982; 59: 439) for the determination of sodium-22 (22Na+) uptake in human erythrocytes. This modification facilitates the separation of 22Na+ taken up by erythrocytes from the free 22Na+ in the buffer by the addition of dibutyl phthalate, which forms an immiscible layer between the two. To further improve the sensitivity of 22Na+ uptake, we incubated a range of known numbers of erythrocytes with 22Na+ as opposed to the single cell suspension of known hematocrit used in Mahoney's et al. procedure (1). Erythrocytes are incubated in KCI buffer containing 2627 Bq (0.071 microCi) 22Na+ in a total volume of 0.5 mL for 0.5 h at 37 degrees C. Incubation is terminated by placing the tubes in ice for 10 min and the amount of 22Na+ taken up by the erythrocytes determined. We observe a linear relationship between erythrocyte concentrations (0.5 to 2.5 X 10(9) cells/mL) and percent uptake of 22Na+ (0.37 +/- 0.06 (1 SD) to 1.85 +/- 0.27 (1 SD) of the total 22Na+, respectively). The procedure is simple and sensitive, and can be used in clinical laboratories for the routine evaluation of 22Na+ uptake in erythrocytes.
我们报告了对马奥尼等人（《血液》，1982年；59：439）技术的一种改进，用于测定人红细胞中钠-22（22Na+）的摄取。这种改进通过添加邻苯二甲酸二丁酯促进了红细胞摄取的22Na+与缓冲液中游离的22Na+的分离，邻苯二甲酸二丁酯在两者之间形成了一个不混溶层。为了进一步提高22Na+摄取的灵敏度，我们用一系列已知数量的红细胞与22Na+一起孵育，而不是像马奥尼等人的方法（1）中使用已知血细胞比容的单细胞悬液。将红细胞在含有2627贝可勒尔（0.071微居里）22Na+的氯化钾缓冲液中，总体积为0.5毫升，在37摄氏度下孵育0.5小时。通过将试管置于冰中10分钟终止孵育，并测定红细胞摄取的22Na+量。我们观察到红细胞浓度（0.5至2.5×10（9）个细胞/毫升）与22Na+摄取百分比（分别占总22Na+的0.37±0.06（1个标准差）至1.85±0.27（1个标准差））之间存在线性关系。该方法简单且灵敏，可用于临床实验室对红细胞中22Na+摄取的常规评估。
