一种用于蛋白质标记和时间分辨荧光应用的新型铕螯合物。

A new europium chelate for protein labelling and time-resolved fluorometric applications.

作者信息

Evangelista R A, Pollak A, Allore B, Templeton E F, Morton R C, Diamandis E P

机构信息

HSC Research Development Corporation, University of Toronto, Ontario, Canada.

出版信息

Clin Biochem. 1988 Jun;21(3):173-8. doi: 10.1016/0009-9120(88)90006-9.

DOI:10.1016/0009-9120(88)90006-9

PMID:3390907

Abstract

Synthetic procedures are presented for a new chelator that forms stable and highly fluorescent complexes with Eu3+. This chelator, 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) is synthesized in a high-yield three-step procedure. BCPDA can be covalently incorporated into proteins under relatively mild conditions, and when complexed with Eu3+ forms a fluorescent product that has a lifetime in the range of 0.4 to 0.7 ms. Thus, it is useful for time-resolved fluorescence immunoassay applications.

摘要

本文介绍了一种新型螯合剂的合成方法，该螯合剂能与Eu3+形成稳定且高荧光的配合物。这种螯合剂，4,7-双(氯磺苯基)-1,10-菲咯啉-2,9-二羧酸(BCPDA)，通过高产率的三步法合成。BCPDA可以在相对温和的条件下共价结合到蛋白质中，当与Eu3+络合时，形成一种荧光产物，其寿命在0.4到0.7毫秒范围内。因此，它可用于时间分辨荧光免疫分析应用。