Shankaran P, Reichstein E, Khosravi M J, Diamandis E P
CyberFluor Inc., Toronto, Ontario, Canada.
J Clin Microbiol. 1990 Mar;28(3):573-9. doi: 10.1128/jcm.28.3.573-579.1990.
We describe new methods for the detection of immunoglobulin G (IgG) and IgM rubella-specific antibodies in serum. The IgG assay was based on a solid-phase rubella antigen immobilization approach, and the IgM assay was based on the IgM capture assay principle. Both assays used biotinylated antibodies (anti-human IgG and antirubella monoclonal antibody, respectively). The tracer system was based on streptavidin labeled with a fluorescent europium chelate. The final measurements were done by using time-resolved fluorescence. Both assays were thoroughly evaluated with clinical samples and compared successfully with established techniques. We anticipate that these assays are suitable for routine clinical use.
我们描述了检测血清中免疫球蛋白G（IgG）和IgM风疹特异性抗体的新方法。IgG检测基于固相风疹抗原固定化方法，IgM检测基于IgM捕获检测原理。两种检测均使用生物素化抗体（分别为抗人IgG和抗风疹单克隆抗体）。示踪系统基于用荧光铕螯合物标记的链霉亲和素。最终测量通过时间分辨荧光进行。两种检测均使用临床样本进行了全面评估，并与现有技术成功进行了比较。我们预计这些检测适用于常规临床应用。
