Bertholf R L, Savory M G, Winborne K H, Hundley J C, Plummer G M, Savory J
Department of Pathology, University of Virginia Medical Center, Charlottesville 22980.
Clin Chem. 1988 Jul;34(7):1500-2.
We evaluated the performance of the lithium ion-selective electrode (ISE) in the Du Pont Na/K/Li analyzer. Lithium concentrations in 106 serum samples from patients being treated with lithium were measured in duplicate with the ISE and by flame photometry. The slope of the regression line for the two methods was 1.004 with a standard error of the estimate of 0.049 mmol/L (x = flame photometry, y = ISE). Lithium measurements by the ISE method in serum or aqueous standards were linear to greater than 2.0 mmol/L. Within-run CVs for low (0.31 mmol/L) and high (1.15 mmol/L) lithium controls were 5.9% and 1.7%, respectively (n = 20). Day-to-day CVs for the same controls were 9.8% and 3.3%, respectively (n = 20). There was no significant interference when the concentrations of sodium, potassium, calcium, or magnesium were varied, nor did intervening urinary lithium analyses affect the measurement of serum lithium. Results for lithium measurement in four serum-based survey materials compared well with results by isotope dilution/mass spectrometry.
我们评估了杜邦钠/钾/锂分析仪中锂离子选择性电极（ISE）的性能。对106份正在接受锂治疗的患者的血清样本中的锂浓度，使用ISE和火焰光度法进行了重复测量。两种方法回归线的斜率为1.004，估计标准误差为0.049 mmol/L（x =火焰光度法，y = ISE）。ISE法对血清或水性标准品中锂的测量在大于2.0 mmol/L时呈线性。低浓度（0.31 mmol/L）和高浓度（1.15 mmol/L）锂对照品的批内变异系数分别为5.9%和1.7%（n = 20）。相同对照品的日间变异系数分别为9.8%和3.3%（n = 20）。当钠、钾、钙或镁的浓度变化时，没有明显干扰，并且介入的尿锂分析也不影响血清锂的测量。四种基于血清的调查材料中锂测量的结果与同位素稀释/质谱法的结果比较良好。
