Li Maoran, Xu Danhua, Xia Xiang, Ni Bo, Zhu Chunchao, Zhao Gang, Cao Hui
Department of Gastrointestinal Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P.R. China.
J Int Med Res. 2021 Apr;49(4):3000605211009802. doi: 10.1177/03000605211009802.
Semaphorin 3C (Sema3C) may regulate tumor metastasis and prognosis. We determined the biological roles of Sema3C in the hepatic metastasis of gastric adenocarcinoma and evaluated its clinical significance as a potential biomarker.
Sema3C expression in gastric cancer (GC) cell lines and tissues was measured using RT-qPCR and western blotting. Moreover, Sema3C functions were analyzed using Transwell assays and metastasis assays in gain- and loss-of-function experiments. Furthermore, the impact of Sema3C on the prognosis of 80 randomly selected patients with GC was investigated by immunohistochemistry. Additionally, the expression of epithelial-mesenchymal transition (EMT) indicators was verified by immunohistochemistry in GC tissues.
Sema3C expression was significantly upregulated in highly metastatic GC cell lines and tissues. Additionally, Sema3C promoted invasion, migration and hepatic metastasis in GC cells. Moreover, Sema3C expression was positively correlated with clinicopathological features in GC and paired hepatic metastatic tissues, and Sema3C expression was an independent prognostic factor. Finally, Sema3C expression was associated with node metastasis, hepatic metastasis and EMT marker expression.
Sema3C may play roles in regulating the EMT and metastasis of gastric adenocarcinoma, highlighting its potential use as a prognostic factor for hepatic metastasis and poor prognosis in gastric adenocarcinoma.
信号素3C（Sema3C）可能调控肿瘤转移及预后。我们确定了Sema3C在胃腺癌肝转移中的生物学作用，并评估了其作为潜在生物标志物的临床意义。
采用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法检测胃癌（GC）细胞系和组织中Sema3C的表达。此外，在功能获得和功能缺失实验中，通过Transwell实验和转移实验分析Sema3C的功能。此外，通过免疫组织化学研究Sema3C对80例随机选择的GC患者预后的影响。另外，通过免疫组织化学在GC组织中验证上皮-间质转化（EMT）指标的表达。
Sema3C在高转移性GC细胞系和组织中的表达显著上调。此外，Sema3C促进GC细胞的侵袭、迁移和肝转移。而且，Sema3C表达与GC及配对肝转移组织的临床病理特征呈正相关，且Sema3C表达是一个独立的预后因素。最后，Sema3C表达与淋巴结转移、肝转移及EMT标志物表达相关。
Sema3C可能在调控胃腺癌的EMT和转移中发挥作用，突出了其作为胃腺癌肝转移和预后不良的预后因素的潜在用途。
