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视黄醇结合蛋白4作为一种潜在的肿瘤生物标志物并促进胃癌的恶性行为。

Retinol Binding Protein 4 Serves as a Potential Tumor Biomarker and Promotes Malignant Behavior in Gastric Cancer.

作者信息

Yu Yantao, Zhang Chenkai, Sun Qiannan, Baral Shantanu, Ding Jianyue, Zhao Fanyu, Yao Qing, Gao Shuyang, Liu Bin, Wang Daorong

机构信息

The Yangzhou School of Clinical Medicine of Dalian Medical University, Yangzhou, 225001, People's Republic of China.

General Surgery Institute of Yangzhou, Yangzhou University, Yangzhou, 225001, People's Republic of China.

出版信息

Cancer Manag Res. 2024 Jul 24;16:891-908. doi: 10.2147/CMAR.S480337. eCollection 2024.

DOI:10.2147/CMAR.S480337
PMID:39072342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11283833/
Abstract

BACKGROUND

Gastric cancer (GC) is a highly phenotypically heterogeneous disease and is caused by a combination of factors. Retinol binding protein 4 (RBP4) is a member of a family of lipid transport proteins that are involved in the transport of substances between cells and play a crucial role in a variety of cancers. However, the expression and role of RBP4 in GC remain unknown.

METHODS

In this study, we explored the expression, prognostic significance, immune microenvironment, drug responsiveness and function of associated signaling pathways of RBP4 in GC using web-based bioinformatics tools. Immunohistochemistry and real-time quantitative PCR were utilized to analyze the tissue and cell expression levels of RBP4. CCK-8, colony formation, EDU incorporation, wound healing and transwell assays were applied to demonstrate the effect of RBP4 on GC cell function. Flow cytometric detection of apoptosis after RBP4 knockdown. Nude mice xenograft model elucidates the role of RBP4 for GC in vivo. Related proteins of the RAS signaling pathway were analyzed by employing Western blot assays.

RESULTS

RBP4 is highly expressed in GC. RBP4 is closely associated with patient survival and sensitivity to a wide range of antitumor agents. Knockdown of RBP4 promoted apoptosis and inhibited cell proliferation, invasion and migration. RBP4 promotes GC tumorigenesis in vivo. Finally, RBP4 modulates the RAS/RAF/ERK axis.

CONCLUSION

RBP4 may promote gastric carcinogenesis and development through the RAS/RAF/ERK axis and is expected to be a novel target for GC treatment.

摘要

背景

胃癌（GC）是一种表型高度异质性的疾病，由多种因素共同导致。视黄醇结合蛋白4（RBP4）是脂质转运蛋白家族的成员，参与细胞间物质转运，在多种癌症中起关键作用。然而，RBP4在GC中的表达及作用尚不清楚。

方法

在本研究中，我们使用基于网络的生物信息学工具，探究了RBP4在GC中的表达、预后意义、免疫微环境、药物反应性及相关信号通路的功能。采用免疫组织化学和实时定量PCR分析RBP4的组织和细胞表达水平。应用CCK-8、集落形成、EDU掺入、伤口愈合和transwell实验来证明RBP4对GC细胞功能的影响。RBP4敲低后通过流式细胞术检测细胞凋亡。裸鼠异种移植模型阐明RBP4在体内对GC的作用。采用蛋白质印迹法分析RAS信号通路的相关蛋白。

结果

RBP4在GC中高表达。RBP4与患者生存及对多种抗肿瘤药物的敏感性密切相关。敲低RBP4可促进细胞凋亡，并抑制细胞增殖、侵袭和迁移。RBP4在体内促进GC肿瘤发生。最后，RBP调节RAS/RAF/ERK轴。

结论

RBP4可能通过RAS/RAF/ERK轴促进胃癌发生发展，有望成为GC治疗的新靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86e3/11283833/ebb942643ef5/CMAR-16-891-g0010.jpg
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本文引用的文献

1
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2
Sema3C promotes hepatic metastasis and predicts poor prognosis in gastric adenocarcinoma.Sema3C促进胃腺癌肝转移并预示不良预后。
J Int Med Res. 2021 Apr;49(4):3000605211009802. doi: 10.1177/03000605211009802.
3
Tumor Immune Microenvironment during Epithelial-Mesenchymal Transition.上皮-间质转化过程中的肿瘤免疫微环境。
Clin Cancer Res. 2021 Sep 1;27(17):4669-4679. doi: 10.1158/1078-0432.CCR-20-4459. Epub 2021 Apr 7.
4
Biological Functions of RBP4 and Its Relevance for Human Diseases.视黄醇结合蛋白4的生物学功能及其与人类疾病的相关性。
Front Physiol. 2021 Mar 11;12:659977. doi: 10.3389/fphys.2021.659977. eCollection 2021.
5
Necroptosis, pyroptosis and apoptosis: an intricate game of cell death.细胞程序性死亡方式：细胞坏死、细胞焦亡与细胞凋亡。
Cell Mol Immunol. 2021 May;18(5):1106-1121. doi: 10.1038/s41423-020-00630-3. Epub 2021 Mar 30.
6
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.《全球癌症统计数据 2020：全球 185 个国家和地区 36 种癌症的发病率和死亡率估计》。
CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.
7
Gastric cancer.胃癌。
Lancet. 2020 Aug 29;396(10251):635-648. doi: 10.1016/S0140-6736(20)31288-5.
8
Targeted Therapies in Advanced Gastric Cancer.晚期胃癌的靶向治疗。
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9
Guidelines and definitions for research on epithelial-mesenchymal transition.上皮-间质转化研究的指南和定义。
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10
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